| 摘要: |
| 目的 研究HBsAg 对脂多糖诱导树突状细胞（DCs）分泌IL-6 和IL-12 的影响。方法 采用重组人粒- 单核
集落刺激因子（rhGM-CSF）联合重组人白细胞介素4（rhIL-4）诱导单个核细胞得到未成熟树突状细胞（iDCs），分别加入HBsAg1、2、5μg/ml，并设对照组，各组再加入脂多糖诱导为成熟DCs。采用流式细胞术鉴定各组iDCs 的表型（CD11c、HLA-DR），ELISA 法检测脂多糖诱导后培养上清液中IL-6 和IL-12 的水平。结果 iDCs 的CD11c/ HLA-DR 双阳性率为（83.62±6.89）%，显著高于单纯培养组（20.57±11.19）%，差异有统计学意义（P＜0.05）。HBsAg 1、2、5μg/ml 组培养上清液中IL-6 分别为（609.36±127.06）、（566.01±173.46）、（295.03±76.08）pg/ml，低于对照组（1356.97±181.78）pg/ml（均P＜0.05）；HBsAg2、5滋g/ml 组IL-12 分别为（854.49±67.92）、（472.09±55.70）pg/ml，低于对照组（1248.78±112.09）pg/ml（均P＜0.05）；1滋g/ml
组IL-12（1103.53±134.15）pg/ml，与对照组比较差异无统计学意义（P＞0.05）。结论 低浓度HBsAg 可下调脂多糖诱导的DCs 细胞因子分泌。 |
| 关键词: 乙型肝炎表面抗原 树突状细胞 IL-6 IL-12 |
| DOI： |
| 分类号: |
| 基金项目:浙江省医药卫生科学研究基金计划项目 |
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| Effect of HBsAg on IL-6/IL-12 production of mononuclear-derived dendritic cells stimulated by lipopolysaccharide |
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TANG Yongzhi,YAN Fei,ZHU Jiansheng,CHEN Huazhong,ZHU Min,XIAO Ming,LIN Xi,SHAO Hui
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Affiliated Taizhou Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the effect of HBsAg on IL-6/IL-12 production of dendritic cells (DCs) stimulated by Lipopolysaccharide (LPS). Methods Mononuclear cells were isolated and cultured in complete medium containing GM-CSF plus IL-4 to obtain immature DCs. Flow cytometry were performed to define the phenotypic characteristics of iDCs. Different concentrations(1, 2 and 5滋g/ml) of HBsAg were added in culture medium. The DCs were cultured for 24 h for maturation, followed by a 24h-differentiation culture with LPS (1滋g/ml). The inflammatory cytokines from cell supernatant were determined by enzyme-linked immunosorbent assay (ELISA). Results The expression of CD11c and HLA-DR on immature DCs was (83.62±6.89) %, compare to (20.57±11.19)%in control group. IL-6 production was significantly decreased with the treatment of HBsAg(1, 2 and 5μg/ml, P＜0.05). IL-12 production demonstrates the similar results with the treatment of 2 and 5滋g/ml HBsAg (P＜0.05), but no changes with the treatment of 1μg/ml HBsAg (P＞0.05). Conclusion HBsAg may down-regulate the secretion of
cytokines in DCs stimulated by LPS at lower dosage. |
| Key words: HBsAg Dendritic cells Interleukin-6 Interleukin-12 |
