| 摘要: |
| 目的 研究5- 脂氧合酶（5-LOX）在恶唑酮诱导溃疡性结肠炎（UC）小鼠结肠黏膜中的表达情况，探讨其在UC 发病
机制中的作用及其与炎症程度的关系。方法 50只Balb/c小鼠分为正常对照组10 只和模型组40只。模型组用3%恶唑酮致敏2d，第5 天用0.5%恶唑酮灌肠。第8 天用颈椎脱臼法处死所有小鼠。实验期间观察小鼠疾病活动指数（DAI），处死小鼠后行结肠组织学病理评分（HPS）。RT-PCR 法检测结肠黏膜5-LOXmRNA 的表达，免疫组织化学方法检测结肠黏膜5-LOX 的蛋白表达情况。结果 模型组DAI 和HPS 均高于对照组，5-LOXmRNA和蛋白表达也明显高于对照组（均P＜0.01），并随炎症程度加重，表达水平明显增高。结论 5-LOX 在UC 中表达水平明显升高，并参与了UC 的发生和发展，在一定程度上可反应疾病的活动度，并且可能作为药物治疗的潜在靶点。 |
| 关键词: 溃疡性结肠炎 5-脂氧合酶 RT-PCR 免疫组织化学 |
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| Expression of 5-lipoxygenase mRNA and protein in colon mucosa of mice with oxazolone-induced ulcerative colitis |
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ZHONG Wane, ZHANG Haifeng, ZHOU Guoxiong
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Affiliated Hospital of Nantong University
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| Abstract: |
| Objective To investigate the expression of 5-lipoxygenase (5-LOX ) mRNA in colon mucosa in mice with
oxazolone-induced ulcerative colitis (UC). Methods Fifty female Balb/c mice were divided into control group (n=10) and UC group (n=40). Mice in UC group received subcutaneous injection of 3% (w/v) oxazolone for 2d and subsequently received enema with 0.5%(w/v) oxazolone on d5 after sensitization. On d7 the mice were sacrificed for harvest of colon samples; disease activity index (DAI) and histopathological score (HPS) were also evaluated. The expression of 5-LOX mRNA and protein in colon mucosa were detected by RT-PCR and immunohistochemistry, respectively. Results The DAI and HPS were higher in UC group than those in control group. The expression of 5-LOX mRNA and protein were significantly increased in UC mice compared to controls(P＜0.01), and the expression was positively correlated with the severity of colonic inflammation. Conclusion The expression of
5-LOX is up-regulated in colon mucosa of mice with oxazolone-induced ulcerative colitis, which indicates that 5-LOX may be involved in the development of UC and 5-LOX might be a potential pharmacological target for treatment of UC. |
| Key words: Ulcerative colitis 5-LOX RT-PCR Immunohistochemistry |
