| 摘要: |
| 目的 观察放射性肺损伤大鼠肺组织转化生长因子-β1（TGF-β1）表达水平及肺组织病理学特征，探讨扶正活血中
药对放射性肺损伤的防治作用及可能机制。方法 将56 只SD 雌性大鼠随机分为模型组、前期干预组、后期短程干预组及后期长程干预组，每组14 只，通过单次大剂量6MV-Ｘ线加速器胸部照射制成放射性肺损伤大鼠模型，分别不用及照射前2 周内、照射后1个月内、照射后3 个月内用扶正活血中药灌胃，于照射后3、6 个月分别随机处死各组大鼠7 只，取大鼠肺组织进行免疫组化检测TGF-β1蛋白表达强度及观察不同时相肺组织病理学特征。结果 各干预组大鼠肺组织TGF-β1蛋白表达强度明显低于模型组，放射性肺损伤病理学改变明显轻于模型组。结论 扶正活血中药具有抑制TGF-β1分泌，有效阻止或延缓肺纤维化的形成，对放射性肺损伤的防治具有一定作用。 |
| 关键词: 放射性肺损伤 扶正活血中药 转化生长因子β1 |
| DOI： |
| 分类号: |
| 基金项目:浙江省中医药攀登工程建设资助项目 |
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| Protective effects of Chinese medicine of Fuzheng Huoxue on radiation-induced lung injury in rats |
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ZHENG Haihong, GAN Meifu, WENG Shouxiang
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Taizhou Enze Medical Center,Taizhou Hospital of Zhejiang Province
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| Abstract: |
| Objective To investigate the effect of Chinese medicine of Fuzheng Huoxue on radiation-induced lung
injury in rats. Methods Fifty six S-D rats were randomly allocated into irradiation group(n=14), early intervention group(n=14),short-term intervention group (n=14), long-term intervention group (n=14). The radiation lung injury was induced by irradiation on chest of rats with a single large dose using 6-MV linear accelerator. Chinese medicine of Fuzheng Huoxue was administrated by gavage at 2 weeks before irradiation(early intervention group), within 1 month or 3 months after irradiation(short-term or long-term intervention groups), respectively. The animals were sacrificed at the end of the 3 and 6 months after irradiation (7 in each batch). The histopathological changes of lungs was observed and the expression of TGF-β1 in lung tissue was detected with immunohistochemistry. Results The expression of TGF-β1 in intervention groups was significantly lower than that in model group. Compared to model group the histopathological changes of radiation-induced lung injury were significantly ameliorated in intervention groups. Conclusion Chinese medicine of Fuzheng Huoxue can alleviate or delay the radiation-induced lung injury in rats, which may be associated with the down-regulation of TGF-β1 expression in lung tissue. |
| Key words: Radiation-induced lung injury Fuzheng huoxue Recipe TGF-β1 |
