| 摘要: |
| 目的分析miR-128在结肠癌细胞中的表达情况，研究其对结肠癌细胞生物学行为的影响及作用机制。方法对转染miR-128模拟物后的结肠癌SW48细胞进行培养，采用MTT法行细胞增殖实验检测细胞增殖能力，细胞划痕法检测细胞迁移能力，Transwell细胞侵袭实验检测细胞侵袭能力；并采用RT-RCR检测miR-128mRNA的表达，Westernblot法检测miR-128蛋白的表达。结果转染miR-128模拟物后的结肠癌SW48细胞较转染空白对照模拟物后的，细胞增殖、侵袭及迁移能力均下降（均P＜0.05），且转录因子Sp1基因在mRNA及蛋白水平均下降（均P＜0.05）。结论miR-128可抑制结肠癌细胞增殖及侵袭转移，可能通过调控Sp1基因发挥上述作用。 |
| 关键词: miR-128 结肠癌 侵袭转移 增殖 |
| DOI： |
| 分类号: |
| 基金项目:宁波市科技计划项目 |
|
| MicroRNA-128 inhibits invasion of colon cancer cells |
|
CAO Yuepeng,PANG Hongshuang,CHEN Cheng,YUAN Danping
|
|
Ningbo First Municipal hospital
|
| Abstract: |
| Objective To investigate the expression of microRNA-128 (miR-128) in colon cancer cells and its effect on invasion and metastasis of cancer cells. Methods Human colon cancer SW48 cells were transfected with miR-128 mimics. The cell proliferation was determined by MTT assay, cell migration and invasion were detected by scratch and Transwell methods, the target genes of miR-128 were identified by Western Blot and qPCR methods. Results The proliferation, invasion and migration in miR-128-transfected SW48 cells (P ＜0.05). The expression of Sp1 mRNA and protein level were decreased in miR-128 over-expressing SW48 cells (P＜0.05). Conclusion MicroRNA-128 can inhibit the proliferation and invasion of colon cancer, in which Sp1 gene may be involved. |
| Key words: miR-128 Colon cancer Metastasis Proliferation |
