| 摘要: |
| 目的 通过观察高糖环境下小鼠足细胞表达NEPH1、desmin、TGF-β1 和Smad7 的变化，探讨高糖损伤足细胞
的机制。方法 将培养成熟的小鼠足细胞用RandA 1.0 软件完全随机分为高糖1、2、3 组（葡萄糖浓度分别为15、25 和30mmol/L）和对照组（葡萄糖浓度5mmol/L），倒置显微镜下观察高糖干预前后足细胞的形态变化，采用RT-PCR 和Western blot技术分别检测足细胞NEPH1、desmin、TGF-β1 和Smad7 的mRNA 和蛋白表达变化。结果 与对照组相比，高糖环境下培养48h 后，足细胞形态发生不同程度变化。NEPH1 和Smad7 的mRNA 表达较对照组显著减少（P＜0.05 或0.01），desmin 和TGF-β1的mRNA 表达较对照组增加（P＜0.05 或0.01）。其中高糖2 组的NEPH1 和Smad7 的蛋白表达较对照组显著减少（均P＜0.01），而desmin 和TGF-β1的蛋白表达较对照组显著增加（P＜0.01）。小鼠足细胞在高糖培养下NEPH1 mRNA 和desmin mRNA 的表达呈显著负相关（r=-0.993，P＜0.01）；NEPH1 mRNA 和TGF-β1 mRNA 的表达呈显著负相关（r=-0.989，P＜0.01）；TGF-β1 mRNA 的表达和Smad7 mRNA 的表达呈显著负相关（r=-0.996，P＜0.01）。结论 高糖可能通过激活TGF-β/Smad信号通路诱导小鼠足细胞发生表型转化。 |
| 关键词: 高糖 足细胞 表型转化 NEPH1 Smad7 |
| DOI： |
| 分类号: |
| 基金项目:浙江省中医药科学研究基金计划；杭州市科委医疗卫生及重点专科专病科研攻关专项 |
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| Impact of high-glucose on epithelial-mesenchymal transition in mouse podocytes |
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YE Xun, HOU Pengchao, HONG Yuzhi
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Hangzhou Traditional Chinese Medicial Hospital
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| Abstract: |
| Objective To investigate the effect of high-glucose on epithelial-mesenchymal transition in mouse
podocytes. Methods Matured mouse podocytes were cultured and randomly divided into control group (5mmol/L D-glucose) and different high glucose groups (15, 25 and 30 mmol/L D-glucose, respectively) by means of RandA1.0 randomized software. Morphological changes of podocytes were examined with inverted microscope and expression changes of NEPH1, desmin, TGF-β1 and Smad7 mRNA and protein were detected by RT-PCR and Western blot, respectively. Results Matured podocytes incubated with high glucose were converted from arborized cells into cobblestone appearance after 48h culture. Compared with the control group, the expression of NEPH1 and Smad7 mRNA in pedocytes cultured with different high glucose were significantly decreased(P＜0.05 or 0.01); meanwhile, the expressions of desmin and TGF-β1mRNA in pedocytes cultured with different doses of high glucose were significantly increased in comparison with those of control group (P＜0.05 or 0.01). The protein expressions of NEPH1 and
Smad7 were significantly decreased while those of desmin and TGF-β1 were significantly increased in 25mmol/L glucose group compared with control group (P＜0.01). The expression of NEPH1 mRNA was negatively correlated with that of desmin mRNA(r=-0.993, P＜0.01) and TGF-β1mRNA(r=-0.989, P＜0.01). The expression of TGF-β1 mRNA was negatively correlated with that of Smad7 mRNA in mouse podocyte exposed to high glucose (r=-0.996, P＜0.01). Conclusion The phenotypic transformation ofmouse podocytes can be possibly inducedby high glucose via activating the TGF-β/Smad signal pathway. |
| Key words: High glucose Podocyte Phenotypic transformation NEPH1 Smad7 |
