| 摘要: |
| 目的观察Stathmin和p27在胃癌演变过程中的表达变化，探讨其与幽门螺杆菌（Hp）的关系。方法选取本院胃癌手术标本90例，胃镜活检标本160例及同期行内镜下黏膜剥离术后病理证实为早期胃癌标本56例，应用免疫组化法检测慢性胃炎黏膜（n=80）、上皮内瘤变（n=80）、早期胃癌（n=56）和进展期胃癌（n=90）组织中Stathmin和p27蛋白的表达水平。用14C呼气试验和组织学改良Giemsa染色方法联合检测胃癌中Hp感染情况，分析Stathmin、p27与Hp感染的关系。结果与胃炎组和上皮内瘤变组相比，进展期胃癌组Stathmin和p27的表达水平差异均存有统计学意义（均P＜0.05）。在上皮内瘤变组与胃炎组Stathmin表达水平差异有统计学意义（P＜0.05），而p27表达水平差异无统计学意义（P＞0.05）。不同胃癌分化类型、浸润深度、TNM分期及淋巴转移与否者Stathmin和p27的表达水平差异均有统计学意义（均P＜0.05）。Hp感染者Stathmin蛋白表达增多，p27蛋白表达下降，均有统计学意义（均P＜0.05)。结论p27联合Stathmin蛋白测定可以早期预测胃癌发生，更可以作为反映胃癌良恶性表型及预后的重要指标。 |
| 关键词: 胃癌 上皮内瘤变 Stathmin p27 幽门螺杆菌 |
| DOI： |
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| Changes of stathmin and p27 expression in evolution of gastric cancer and their relation to infection of Helicobacter pylori |
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HU Guimei, YE Guoliang, QI Lijun
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the First Affiliated Hospital of Ningbo Medical University
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| Abstract: |
| Objective To study the expression of the stathmin and p27 protein in the gastritis, intraepithelial neoplasia and gastric cancer and their relation to Helicobacter pylori (Hp) infection. Methods Immunohistochemical method were employed to detect stathmin and p27 protein expressions in gastritis, gastric intraepithelial neoplasia and gastric cancer specimen. The 14C-urea breath test and methylene blue method were used to detect Hp infection. The correlation of stathmin and p27 expression with Hp infection was analyzed. Results Compared with gastritis and gastric intraepithelial neoplasia, the expression
of the stathmin and p27 in the gastric cancer were significantly increased (P＜0.05). There was significant difference in stathmin
expression between gastritis and intraepithelial neoplasia, but no difference in p27 expression between two groups. The expression of the stathmin and p27 protein was correlated with degree of differentiation, depth of invasion, lymph node metastasis, and TNM stage of gastric carcinoma. The expressions of stathmin and p27 were significantly correlated with Hp infection (P＜0.05). Conclusion Stathmin and p27 may be involved in the pathogenesis and progression of gastric cancer, indi- cating they might bea potential target for screening of early gastric cancer. |
| Key words: Gastric cancer Intraepithelial neoplasia Stathmin p27 H.pylori |
