| 摘要: |
| 目的 研究多梳基因Bmi-1 在甲状腺乳头状癌组织中的表达及临床意义。方法 分别采用免疫组化法、Westernblot 法和RT-PCR 法检测30 例甲状腺乳头状癌组织、30 例结节性甲状腺肿组织及30 例正常甲状腺组织中多梳基因Bmi-1 及其mRNA 的表达情况，并分析Bmi-1与甲状腺乳头状癌临床病理特征的关系。结果 免疫组化法检测发现Bmi-1 在甲状腺乳头状癌组织中阳性表达率73.3%，与结节性甲状腺肿（36.7%）及与正常组织（16.7%）的阳性表达率相比差异有统计学意义（P＜0.05）。Western blot 法检测甲状腺乳头状癌中Bmi-1 表达量与结节性甲状腺肿组织及正常组织相比，差异有统计学意义（P＜0.05）。RT-PCR法检测甲状腺乳头状癌中Bmi-1mRNA 表达量与结节性甲状腺肿组织及正常组织相比，差异有统计学意义（P＜0.05）。Bmi-1 阳性表达在甲状腺乳头状癌有无淋巴结转移中的差异有统计学意义（P＜0.05），在年龄、性别、肿瘤大小、分期等差异无统计学意义。结论 Bmi-1 在甲状腺乳头状癌中表达升高，可能在甲状腺乳头状癌的发生、发展过程中起重要作用，并可望作为甲状腺乳头状癌临床诊断的标记物之一。 |
| 关键词: Bmi-1 甲状腺乳头状癌 RT-PCR Western blot 免疫组化法 |
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| 基金项目:杭州市医药卫生科技计划项目；杭州市重大科技创新专项项目 |
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| Expression of Bmi-1 mRNA and protein in papillary thyroid carcinoma and its significance |
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PAN Gang, XU Xiaocheng, ZHANG Yu, PENG You, ZHANG Wo, DING Jinwang, LUO Dingcun
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Huzhou First People’s Hospital
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| Abstract: |
| Objective To investigate the expression and clinical significance of Bmi-1 in papillary thyroid carcinoma. Methods The expression of Bmi-1 mRNA and protein was detected by RT-PCR, Western blot and immunohistochemistry in 30 samples of papillary thyroid carcinomas, 30 samples of nodular goiters and 30 samples of normal thyroid tissue, respectively. The correlation of Bmi-1 expression with clinicopathological features of patients was also analyzed. Results The results of immunohistochemistry showed that the positive rates of Bmi-1 protein in papillary thyroid carcinomas，nodular goiters， normal thyroid tissues were 73.3%, 36.7% and 16.7%, respectively(P＜0.05). Western blot showed that the expression of Bmi-1 protein in papillary thyroid carcinomas was significantly higher than those in nodular goiter tissue and normal tissue(P＜ 0.05). The expression of Bmi-1mRNA in papillary thyroid carcinomas was significantly higher than those in nodular goiters and normal thyroid tissues (P＜0.05). Bmi-1 expression was closely relevant to lymph node metastasis (P＜0.05), but not to gender, age and differentiation degree. Conclusion The expression of Bmi-1 gene is up-regulated in papillary thyroid carcinomas, indicating that Bmi-1 may be involved in the development of papillary thyroid carcinoma and may serve as a marker for clinical diagnosis. |
| Key words: Bmi-1 Papillary thyroid carcinoma RT-PCR Western blot Immunohistochemistry |
