| 摘要: |
| 目的 探讨腰椎Modic I型改变的腰椎终板与椎间盘髓核中TNF-α、IL-6、IL-8 的相关性。方法 收集30 例因下腰痛入院手术治疗并行腰椎MRI 检查确认为腰椎Modic I型改变患者的术中病灶椎间盘标本，进行免疫组化检测，并观察椎间盘组织病理变化及炎症因子的表达，IL-6、IL-8 相对表达水平以相对表达量△Ct 或（2-△Ct×104）进行分析。结果 Modic I型终板中TNF-α表达水平（9.2±0.7）高于髓核（7.2±0.5），差异有统计学意义（P＜0.05）；髓核中△Ct IL-6、△Ct IL-8 相对表达量（6.34±1.82、10.62±1.88）高于终板（4.07±1.86、5.92±1.31），差异均有统计学意义（均P＜0.05）。结论 Modic I型终板中TNF-α、IL-6、IL-8的表达水平明显高于髓核，其引起下腰痛的原因主要为终板的炎症改变。 |
| 关键词: Modic I型 炎症因子 腰椎间盘 终板 |
| DOI： |
| 分类号: |
| 基金项目:浙江省自然基金资助项目 |
|
| Expression of TNF-α, IL-6 and IL-8 in lumbar vertebral nucleus pulposus and end plate tissue in patients with Modic type Ichanges |
|
ZHANG Rujie,CHEN Guanjun,ZHUNG Wei,CHENG Yan,TONG Peijian
|
| Abstract: |
| Objective To investigate the expression of TNF-α, IL-6 and IL-8 in lumbar vertebral nucleus pulposus and end plate tissue in patients with Modic type I changes. Methods Thirty patients with low back pain and MRI-confirmed lumbar end-plate Modic type I changes underwent surgical treatment. The disc specimens were collected in the surgery and examined with Immunohistochemistry for expression of TNF-α, IL-6 and IL-8. The association of pathological changes of intervertebral disc tissue with the expression of TNF-α, IL-6, IL-8 and the relative expression of Ct or(2-△Ct×104) was analyzed. Results The expression level of TNF-α in lumbar end plates was higher than that in nucleus pulposus (9.2 ±0.7, 7.2 ± 0.5, P＜0.05); while the expressions of IL-6 and IL-8 in nucleus was higher than those in end-plate (6.34 ±1.82, 4.07±1.86 and 10.62±1.88, 5.92± 1.31, respectively, P＜0.05). Conclusion The expression of inflammatory factors in lumbar endplate were above that of nucleus. The low back pain was caused by the inflammation changes in lumbar end plate. |
| Key words: Modic I type Inflammatory factor Lumbar intervertebral disc Lumbar end plate |
