| 摘要: |
| 目的 研究胰高糖素样肽-1 受体激动剂艾塞那肽对2型糖尿病大鼠糖脂代谢和胰岛细胞的影响及其作用机制。方法 健康雄性SD 大鼠随机分为正常对照组（C 组6只）、正常对照+ 艾塞那肽处理组（C+E 组6 只）、糖尿病组（D 组5 只）以及糖尿病+ 艾塞那肽处理组（D+E 组5 只），由高脂饮食加小剂量STZ 诱导成2型糖尿病后，D+E 组以及C+E 组大鼠予艾塞那肽，5μg，腹部皮下注射，1 次/d 干预；8 周后，分别测定各组大鼠体重、FBG、空腹胰岛素（FINS）、稳态模型胰岛素抵抗指数（HOMA-IR）、胰岛素敏感指数（ISI）以及血脂谱。同时行透射电子显微镜观察大鼠胰岛组织超微结构改变。结果 D+E 组大鼠FBG、FINS、TG、TC、LDL、HOMA-IR水平均明显低于未予干预的D 组大鼠（均P＜0.05），ISI 则显著高于D 组大鼠（P＜0.05）。超微结构研究，与D 组大鼠比较，D+E 组大鼠胰岛β 细胞数量增加，形态较规则，胞质分泌颗粒增加，颗粒密度较正常；仅见少量线粒体肿胀，结构模糊。内质网结构无明显破坏。结论 艾塞那肽能显著改善2型糖尿病大鼠糖脂代谢紊乱，对其残存的胰岛细胞具有明确的保护作用。 |
| 关键词: 糖尿病 胰高糖素样肽-1 受体激动剂 胰岛细胞 超微结构 |
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| 基金项目:浙江省中医药优秀青年人才基金项目 |
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| Effects of exenatide on glucose and lipid metabolism and ultrastructure of islet cells in type 2 diabetic rats |
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WU Hui,XIA Fangzhen,HUA Yanyin,LU Xingli
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Zhejiang Provincial People’s Hospital
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| Abstract: |
| Objective To investigate the effects of glucagon-like peptide-1 agonist exenatide on glucose and lipid metabolismand ultrastructure of islet cells in type 2 diabetic rats. Methods Twenty-two male SD rats were randomly divided into non-diabetic control group (group C, n=6); non-diabetic + exenatide group (group C+E, n=6); diabetic group (group D, n=5) and diabetic + exenatide group (group D+E, n=5). Type 2 diabetes was induced by high-fat diet and low-dose streptozotocin in rats. The rats in C+E group and D+E group were treated with exenatide (5μg/d ) by subcutaneous injection, while the rats in C group and D group were injected with the same volume of normal saline. After 8 weeks, the body weight, fasting blood glucose, insulin, homeoestasis model assessment (HOMA-IR), insulin sensitivity index (ISI) and blood lipid profile were determined in all groups. The ultrastructure of islet cells was observed with transmission electron microscopy. The one-way ANOVAs followed by a Student-Newman-Keuls post hoc test for multiple comparisons were used for statistics. Results Compared with group D, the fasting blood glucose, insulin, triglyceride, total cholesterol, lower density lipoprotein levels, and HOMA-IR were significantly decreased (all P＜0.05), while ISI significantly increased (P＜0.05) in the D+E group. Electron microscopy observation revealed that exenatide attenuated the swollen mitochondria and endoplasmic reticulum in islet cells of the diabetic rats. Conclusion The glucagon-like peptide-1 agonist exenatide can significantly improve glucose and lipid metabolism and protect islet cells in type 2 diabetic rats. |
| Key words: Diabetes Glucagon like peptide 1 agonist Islet cells Ultrastructure |
