| 摘要: |
| 目的 探讨第187位苏氨酸（Thr187）磷酸化的细胞周期素依赖性激酶抑制蛋白（p27kip1，简称p27）（P-p27Thr187）在结直肠癌发生、发展中的作用及其与结直肠癌临床病理特征的关系。方法 收集行手术切除的80例结直肠癌组织标本，以及6对新鲜结直肠癌和相应癌旁正常组织标本；采用免疫组织化学EnVision二步法和蛋白质印迹法检测结直肠癌组织及相应癌旁正常组织中P-p27Thr187和p27的表达；并分析其与结直肠癌临床病理特征的关系。结果免疫组织化学和蛋白质印迹结果均显示，P-p27Thr187在结直肠癌组织中的阳性表达率明显高于癌旁正常组织；结直肠癌组织中P-p27Thr187阳性表达率为100.0%（80/80），高表达者52例（65.0%，52/80），低表达者28例（35.0%，28/80）；而癌旁正常组织中P-p27Thr187阳性表达率为13.8%（11/80），与结直肠癌组织比较差异有统计学意义（P＜0.05）；p27在结直肠癌组织中的阳性表达率为48.8%（39/80），癌旁正常组织中为90.0%（72/80），两组比较差异有统计学意义（P＜0.05）；P-p27Thr187阳性表达与肿瘤细胞分化程度和TNM分期有关（均P＜0.05）；P-p27Thr187表达强度与细胞增殖指标Ki-67表达强度呈正相关（r=0.487，P＜0.05），与p27表达强度呈负相关（r=-0.394，P＜0.05）。结论结直肠癌组织中P-p27Thr187的异常表达可能参与了结直肠癌的发生、发展过程，其有助于综合评估结直肠癌的生物学行为，对结直肠癌的预后判断及诊治具有一定的参考价值。 |
| 关键词: 结直肠肿瘤 p27kip1 磷酸化 临床病理特征 |
| DOI： |
| 分类号: |
| 基金项目:浙江省医学重点学科建设计划）；嘉兴市科技计划项目；嘉兴市第一医院“启明星”计划 |
|
| Expression of Thr187 phosphorylated p27kip1 protein in patients with colorectal cancer and its clinical significance |
|
GUO Zhiqin,LV Qingshan,YUAN Linna,ZHANG Yanping,WU Wanxin
|
|
Jiaxing First People's Hospital
|
| Abstract: |
| Objective To investigate the expression of Thr187 phosphorylated p27kip1 (P-p27Thr187) protein in col- orectal cancer and its clinicopathological significance. Methods The expression of P-p27Thr187 and p27 was detected by immunohistochemical EnVision method and Western blot in 80 specimens of colorectal cancer tissue and 6 paired specimens of colorectal cancer and adjacent normal tissue, Results The positive expression rates of P-p27Thr187 in colorectal cancer tissue was 100.0% (80/80), including high expression in 52 cases (65.0%, 52/80), low expression in 28 cases (35.0%, 28/80); while the
positive expression rates in adjacent normal tissue was 13.8% (11/80) (P＜0.05). The positive expression rates of p27 in colorectal
cancer and adjacent normal tissue were 48.8%(39/80) and 90.0%(72/80), respectively (P＜0.05). The expression of P-p27Thr187 was correlated with tumor differentiation and TNM stage (all P＜0.05); and its expression was positively correlated with prolifera- tion index Ki-67(r= 0.487, P＜0.05), and negatively correlated with p27(r=-0.394, P＜0.05). Conclusion The abnormal expres-
sion of P-p27Thr187 may be involved in the development and progression of colorectal cancer, it might be used as a novel diag- nostic marker and therapeutic target in patients with colorectal cancer. |
| Key words: Colorectal cancer p27kip1 Phosphorylation Clinicopathological features |
