| 摘要: |
| 目的观测类风湿关节炎（RA）患者外周血CD4+T细胞表面程序性死亡因子1（PD-1，CD279）表达的变化、探讨PD-1在RA发生、发展中的作用。方法应用流式细胞术检测40例RA患者和20例健康体检者外周血CD4+T细胞表面PD-1的阳性率，对比分析RA患者病情活动组与稳定组间PD-1表达的差异，分析PD-1阳性率与C反应蛋白（CRP），红细胞沉降率（ESR），类风湿因子（RF）等临床指标的相关关系。结果病情活动期RA患者外周血CD4+T细胞PD-1阳性率为（32.90±12.15）%，明显高于健康体检者（22.32±6.04）%，差异有统计学意义（P＜0.01）；稳定期RA患者的PD-1阳性率为（27.49±6.79）%，与健康体检者的差异无统计学意义（P＞0.05）。RA患者中，CD4+T细胞表面PD-1阳性率与RF呈正相关（r=0.525，P＜0.01），与ESR呈正相关（r=0.328，P＜0.05），与CRP无明显相关性（r=0.232，P＞0.05）。结论病情活动期RA患者外周血CD4+T细胞表面PD-1表达增加，可能对疾病的发生、发展具有重要意义。 |
| 关键词: 类风湿关节炎 程序性死亡因子 1 CD4 阳性 T 细胞 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Expression and significance of programmed death-1 on peripheral CD4+ T cells in patients with rheumatoid arthritis |
|
HU Xiaolei, DING Wenjie, ZHAO Zhigang
|
|
Zhejiang Lishui Central Hospital
|
| Abstract: |
| Objective To investigate the expression of programmed death-1 (PD-1, CD279) on CD4+ T cells from the peripheral blood of patients with rheumatoid arthritis(RA) and its clinical significance. Methods The expression of PD-1 on CD4+ T cells was detected by flow cytometry in 40 RA patients and 20 healthy subjects. Its relationships with C-reaction protein (CRP), erythrocyte sedimentation rate (ESR), rheumatoid factor (RF) were analyzed. Results The positive rate of PD-1 in active RA group was significantly higher than that in normal control (32.90 ±12.15% vs. 22.32 ±6.04%, P ＜0.01), while there was no significant difference between stable RA patients(27.49±6.79%) and normal control(P ＞0.05). The positive rate of PD-1 in active RA patients was correlated positively with RF (r=0.525, P＜0.01) and ESR (r=0.328, P＜0.05), but not with CRP (r=0.232, P ＞ 0.05). Conclusion The expression of PD-1 on CD4+ T cells from the peripheral blood of patients with active RA is up-regulated, which might be involved in the development of RA. |
| Key words: Rheumatoid arthritis Programmed death-1 CD4-positive T-lymphocyte |
