| 摘要: |
| 目的 探讨去甲斑蝥素对人胰腺癌PANC-1细胞凋亡的作用及其机制。方法 将PANC-1 细胞株随机分为处理组
和对照组，处理组加入不同浓度的去甲斑蝥素培养24h后，CCK-8 法检测细胞增殖情况；流式细胞术分析细胞的凋亡情况；免疫印迹法检测细胞内质网应激和凋亡相关蛋白的表达；荧光定量PCR 技术检测细胞内质网应激和凋亡相关蛋白mRNA表达。结果 不同浓度去甲斑蝥素处理PANC-1细胞24h后，与对照组相比，能降低细胞的存活率并诱导其凋亡。与对照组相比，处理组中内质网应激和凋亡相关蛋白的表达水平均上调（均P＜0.05），同时其mRNA 的表达水平亦均上调（均P＜0.05）。结论 去甲斑蝥素能明显抑制人胰腺癌PANC-1 细胞的生长并诱导其凋亡，并且具有浓度依赖性，这一作用可能是通过内质网应激介导的凋亡途径实现的。 |
| 关键词: 胰腺癌 去甲斑蝥素 凋亡 内质网应激 |
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| Effect of norcantharidin on human pancreatic cancer PANC-1 cells |
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ZHANG Riyuan,LIN Shengzhang,CHEN Chong,WANG Yingying,XU Xianzhou
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the Second Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the effect of norcantharidin on human pancreatic cancer PANC-1 cells. Methods PANC-1 cells were cultured with 0.1%DMSOand norcantharidin (0, 10, 50, 75, 100, 150, 200滋mol/L) for 24h. The growth inhibitory effect was observed by using CCK-8, cell apoptosis was determined by flow cytometry (FCM). The expressions of endoplasmic reticulumstress (ERS) markers were detected by Western blot, expressions of GRP78 and GADD153 mRNA were detected by real-time PCR after exposure to norcantharidin. Results CCK-8 assay showed that norcantharidin significant inhibited the growth of PANC-1 cells. Apoptosis rates of PANC-1 cells treated with norcantharidin were significantly higher than those of control group. Western blot analysis showed that norcantharidin up-regulated ERS markers (P＜0.05). Real-time PCR showed that the expression of GRP78 and GADD153 mRNA in PANC-1 cells treated with norcantharidin was higher than that of control group (P＜0.05). Conclusion Norcantharidin can inhibit the growth of human pancreatic cancer PANC-1 cells and induce cell apoptosis.ERS-mediated apoptosis pathway may be involved in norcantharidin-induced apoptosis in pancreatic cancer PANC-1 cells. |
| Key words: Pancreatic cancer Norcantharidin Apoptosis Endoplasmic reticulumstress |
