| 摘要: |
| 目的 从代谢组学角度分析并寻找2型糖尿病（T2DM）患者可能的代谢标记物。方法 选取30例初诊、或有糖尿病史经药物治疗控制不理想且无并发症的T2DM 患者，另选取30例性别、年龄匹配的健康者为正常对照。收集清晨空腹中段尿，以气相色谱- 质谱联用（GC-MS）技术对尿液样本进行代谢图谱分析，正交偏最小二乘法判别研究尿液内源性化合物在两组间的差异。结果 T2DM 组和正常对照组尿液代谢谱明显分离。与正常对照组比较，T2DM 组尿液2,3,4- 三羟基丁酸、肌醇、D- 葡萄糖、D- 葡萄糖酸及尿素含量升高（P＜0.05 或0.01），马尿酸含量减少（P＜0.01）。结论 代谢组学检查提示T2DM 患者尿液中代谢标志物为2，3，4- 三羟基丁酸、肌醇、马尿酸、D-葡萄糖、D- 葡萄糖酸及尿素，观察这些标志物含量的变化有助于T2DM 的临床诊断及发病机制研究。 |
| 关键词: 代谢组学 2型糖尿病 气相色谱- 质谱联用 正交偏最小二乘法判别 |
| DOI： |
| 分类号: |
| 基金项目:浙江省钱江人才计划 |
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| Urinary metabonomics in type 2 diabetic patients |
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SU Junmei,GE Weihong,XU Guangyan,ZHANG Yu
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Zhejiang University of Traditional Chinese Medicine
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| Abstract: |
| Objective To investigate the urinary metabonomics in patients with type 2 diabetes mellitus. Methods Thirty patients with type 2 diabetes mellitus were enrolled as study group, and 30 health subjects served as controls. The morning midstreamurine samples were collected. Gas chromatography-mass spectrometry(GC-MS) was used to analyzemetabonomics of the urine samples.Orthogonal partial least-squares discriminant analysis(OPLS-DA) was appliedto identify potential biomarkers. Results The difference of metabonomics spectra was observed between diabetic patients and normal controls. Compared with the normal controls, the contents of 3-hydroxybutyrate, inositol, D-glucose, D-gluconic acid, urea were increased (P＜0.05 or P＜0.01） and hippuric acid decreased significantly (P＜0.01) in type 2 diabetic patients. Conclusion The 3-hydroxybutyrate, inositol, hippuric acid, D-glucose, D-gluconic acidandurea in the urinemight be the candidate biomarkers for type 2 diabetes mellitus. |
| Key words: Metabonomics Type 2 diabetes mellitus Gas chromatography-mass spectrometry Orthogonal partial least -squares discriminant analysis |
