| 摘要: |
| 目的 探讨神经生长因子（NGF）转染的人脐带间充质干细胞（hUC-MSCs）减轻中枢神经系统功能损伤和促进中枢神经系统功能恢复的可行性。方法 构建缺血、缺氧脑损伤的Wistar 大鼠模型，同时分离培养hUC-MSCs，并将腺病毒介导的NGF转染hUC-MSCs。将转染成功后的hUC-MSCs移植入大鼠模型中，饲养4 周后采用足印分析试验、平衡木试验分别对大鼠的运动功能进行评价。结果 流式细胞仪检测提示hUC-MSCs 分离培养成功，hUC-MSCs 可过表达NGF 蛋白。与hUC-MSCs 组比较，NGF-hUC-MSCs 组幼鼠右侧足印步态重复间距和平衡木通过时间均有统计学差异（均P＜0.05），左侧足印步态重复间距无统计学差异（P＞0.05）。结论 NGF 联合hUC-MSCs 移植能促进实验大鼠的运动功能恢复，为中枢神经系统损伤修复和并发症预防提供了新的途径。 |
| 关键词: 人脐带间充质干细胞 神经生长因子 中枢神经损伤 |
| DOI： |
| 分类号: |
| 基金项目:浙江省温岭市科技计划项目 |
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| Human umbilical cord mesenchymal stem cells transfected with nerve growth factor promote injury recovery of central nerves in rats |
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PENG Yujiang, LI Huiyong, HE Xijun
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the First People’s Hospital of Wenling
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| Abstract: |
| Objective To investigate the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) transfected with nerve growth factor (NGF) on injury recovery of central nerves in rats. Methods The isolated and cultured hUC-MSCs were transfected with adenovirus-mediated NGF gene. Ischemia hypoxia model of central nervous system was induced in Wistar rats. The NGF-transfected hUC-MSCs were transplanted in the model rats. The motion function of rats was evaluated with footprint test and balance beam test. Results The transfected hUC-MSCs successfully expressed NGF protein. The footprint test and balance beamtest showed that NGF-transfected hUC-MSCs promoted motor function recovery in rats with ischemic injury of central nerves. Conclusion NGF-transfected hUC-MSCs can improve damage repair of central nerve in rats. |
| Key words: Human umbilical cord mesenchymal stemcells Nerve growth factor Central nerve damage |
