| 摘要: |
| 目的探讨铜绿假单胞菌对质粒介导喹诺酮类耐药（PMQR）机制，为控制其耐药性传播提供依据。方法筛选住院患者对环丙沙星耐药的69株铜绿假单胞菌，PCR筛选质粒介导的喹诺酮类耐药基因[qnr，aac（6'）-Ib-cr]，阳性产物进行DNA测序，接合转移试验验证PMQR基因。结果69株受试株中，12株aac（6'）-Ib-cr基因阳性，经DNA测序、BLAST比对，3株携带aac（6'）-Ib-cr基因（含有Trp-102→Arg和Asp-179→Tyr突变），另有3株为aac（6'）-Ib野生型；质粒接合转移试验结果阴性；未检出qnr基因。结论铜绿假单胞菌PMQR基因以aac（6'）-Ib-cr基因为主，未检出qnr基因，aac（6'）-Ib-cr基因介导低水平耐药。 |
| 关键词: 铜绿假单胞菌 质粒介导喹诺酮耐药 环丙沙星 |
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| Identification of multidrug resistance genes in quinolone-resistance Pseudomonas aeruginosa with plasmid conjugation method |
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Li Mei,Wang Wenxiang,Shen Yuefei,Pan Fafen
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the First Xiaoshan People's Hospital
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| Abstract: |
| Objective To identify multidrug resistance genes in quinolones resistance-Pseudomonas aeruginosa (PMQR). Methods Sixty nine clinical isolated of ciprofloxacin-resistant Pseudomonas aeruginosa were collected. Quinolone resistance genes [qnr, aac (6 ')- Ib-cr] were screened with PCR plasmid-mediated method, positive products were DNA sequenced, and PMQR genes were verified with plasmid conjugation method. Results Among 69 strains tested, 12 were aac (6 ')- Ib-cr gene positive shown by DNA sequencing, BLAST comparison, including3 contained mutated aac (6') - Ib-cr gene (Trp-102 → Arg and Asp-179 → Tyr), and another 3 carried wild type aac (6 ')- Ib; while plasmid conjugation test was negative and qnr gene was not detected. Conclusion The clinical isolates of Pseudomonas aeruginosa are aac (6 ') - Ib-cr -based low-level resistance PMQR, and qnr gene is not detected. |
| Key words: Pseudomonas aeruginosa Plasmid-mediated quinolone resistance Ciprofloxacin |
