| 摘要: |
| 目的探讨血红素加氧酶-1（HO-1）与一氧化碳（CO）在喘息性疾病婴幼儿血清中含量变化及其临床意义。方法收集112例4岁以下喘息患儿，根据《儿童社区获得性肺炎管理指南（2013修订）》分为重症组（存在低氧血症）49例和轻症组（无低氧血症）63例；选择同期同年龄段急性支气管炎患儿58例为对照组。采用双抗体夹心ELISA技术测定患儿血清HO-1含量，应用双波长分光光度法测定患儿血中碳氧血红蛋白（COHb）含量，进行组间比较分析。结果急性期（入院时）3组间HO-1和COHb含量差异均有统计学意义（均P＜0.01），两两比较，仅重症组（2577.01±879.47）ng/L、（3.84±0.87）%明显高于轻症组（1382.90±731.80）ng/L、（1.17±0.60）%和对照组（1113.73±606.25）ng/L、（1.01±0.54）%（均P＜0.01）。重症组HO-1和COHb含量，均为急性期高于恢复期（均P＜0.01）。急性期重症组HO-1与COHb含量呈正相关（r=0.340，P＜0.05）。结论HO-1和CO可能参与婴幼儿喘息性疾病发展过程，急性重症期两者呈正相关，具有诊断疾病、判断严重程度与预后等临床意义。 |
| 关键词: 喘息性疾病 血红素加氧酶 -1 一氧化碳 婴幼儿 |
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| 基金项目:杭州市科技局科技计划项目；浙江省医药卫生科技计划 |
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| Implication of blood heme oxygenase-1 and carboxyhemoglobin levels in infantile wheezing diseases |
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Wang Kai,Wei Yi,Xu Ting,Lin Xianyao,Chen Dong
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Wenzhou Medical University
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| Abstract: |
| Objective To investigate the implication of blood heme oxygenase-1 (HO-1) and carboxyhemoglobin (COHb) levels in infantile wheezing diseases. Methods One hundred and twelve infants aged ＜3 years with wheezing diseases and 58 infants with acute bronchitis (control group) were enrolled in this study. According to the guidelines for management of childhood community acquired pneumonia (2013 version) , the patients were classified as severe wheezing group (n=49) and mild wheezing group (n=63)．The serum HO-1 were measured by ELISA, the blood COHb levels were measured by dual-wavelength spectrophotometry. Results The HO-1 activities and COHb levels in acute stage of severe wheezing group were markedly higher than those of the mild wheezing group and the control group (P＜0.01), there were no significant difference in HO-1 and COHb levels between mild wheezing group and control group. Compared with the convalescent stage, the HO-1 activities and COHb levels were significantly higher in acute stage of severe wheezing group (P＜0.01), and there were no statistical difference in HO -1 activities and COHb levels between acute stage and convalescent stage in mild wheezing group and control group (P＜ 0.05). The HO-1 and COHb were correlated in acute stage of severe wheezing group (r=0.340, P＜0.05). Conclusion With the wheezing disease progressing, the blood HO-1 and COHb increase, indicating that these two parameters may be used for judgment of disease severity. |
| Key words: Wheezing disease Heme oxygenase-1 CO Infant |
