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卵巢癌患者外周血调节性T细胞的变化及其临床意义
吴杰1, 骆骥才2, 陈文虎1, 许志良3, 张剑英1, 张毅敏1, 金元1, 刘鹏1, 徐笑红1
1.浙江省肿瘤医院检验科;2.浙江大学医学院附属第一医院检验科;3.中国科学院（杭州）生命科学院
摘要:
目的探讨卵巢癌患者外周血调节性T细胞（Treg）水平变化的意义及其临床意义。方法采用流式细胞术测定130例卵巢癌患者和50例体检健康女性的外周血中CD4+、CD8+、NK以及Treg等不同淋巴细胞亚群的比例，比较两组间的差异，分析卵巢癌不同临床病理因素患者Treg水平的差异，并比较手术和化疗患者手术前后或化疗前后Treg水平的变化。结果卵巢癌组外周血CD4+、NK细胞比例以及CD4+/CD8+比值均明显低于正常对照组，而Treg细胞比例明显高于正常对照组；淋巴结转移卵巢癌患者其Treg水平高于淋巴结未转移，且在不同的FIGO分期中III、IV期患者的Treg细胞比例要高于I、II期，差异均有统计学意义（均P＜0.05）；手术后Treg细胞比例较手术前明显下降，差异有统计学意义（P＜0.05）；化疗后Treg细胞比例与化疗前的差异无统计学意义（P＞0.05）。结论卵巢癌患者外周血Treg水平与卵巢癌的分期和进展有关，在卵巢癌预后判断以及疗效监测中有重要意义。
关键词:  调节性 T 细胞 卵巢癌 流式细胞术
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基金项目:浙江省卫生厅资助项目
Regulatory T cell levels in peripheral blood of patients with ovarian cancer and its clinicopathological significance
Wu Jie,Luo Jicai,Chen Wenhu,Xu Zhiliang,Zhang Jianying,Zhang Yimin,Jin Yuan,Liu Peng,Xu Xiaohong
Zhejiang Carcer Hospital
Abstract:
Objective To investigate the levels of CD4+CD25+CD127low regulatory T cells in peripheral blood of patients with ovarian cancer and its relation to clinicopathological features of the disease. Methods Plasma levels of CD4, CD8, NK and regulatory T cells were measured in 130 ovarian cancer patients and 50 healthy controls by flow cytometry. The relation between the plasma levels of regulatory T cells and clinicopathologic features of the disease was analyzed, and changes of regulatory T cells levels in 30 ovarian cancer patients before and after surgery and chemotherapy were compared. Results The percentages of CD4+, NK and CD4+/CD8+ ratio in ovarian cancer group were significantly lower than those in control group. The percentage of regulatory T cells in ovarian cancer group was significantly higher than that in controls; the percentage of regulatory T cells in patients with lymphatic metastasis was significantly higher than that in those without lymphatic metastasis; and the percentage of regulatory T cells in advanced cancer patients was significantly higher than that in early cancer patients (P＜0.05). The levels of regulatory T cells dropped significantly after surgery (P＜0.05), but had no significant changes after chemotherapy (P ＞0.05). Conclusion The results indicate that regulatoryT cells may be a valuable marker for prognosis and treatment monitoring in patients with ovarian cancer.
Key words:  Regulatory T cells Ovarian cancer Flow cytometry