| 摘要: |
| 目的研究术前射频热疗联合FOLFOX辅助治疗对结肠癌组织中微血管密度（MVD）以及凋亡相关分子表达的影响。方法将2013年1月至2014年6月的120例结肠癌患者纳入研究，依据辅助治疗不同分为两组，观察组患者术前接受射频热疗联合FOLFOX化疗，对照组仅接受FOLFOX化疗。采用免疫组化染色的方法检测肿瘤组织中的MVD，Real-timePCR和Westernblot检测肿瘤组织中凋亡相关分子的表达情况。结果观察组患者肿瘤组织中MVD低于对照组（P＜0.05）；观察组患者肿瘤组织中Caspase-3、p16、Bax的mRNA和蛋白含量均高于对照组（均P＜0.05）；观察组患者肿瘤组织中突变型p53基因以及CDC25A、Bcl-2的mRNA和蛋白含量均低于对照组（均P＜0.05）。结论术前射频热疗联合FOLFOX辅助治疗有助于降低结肠癌组织中的MVD，同时上调促凋亡分子、抑制抗凋亡分子的表达。 |
| 关键词: 结肠癌 射频热疗 微血管密度 细胞凋亡 凋亡调控基因 |
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| Effect of preoperative radio-frequency thermotherapy combined with FOLFOX adjuvant treatment on microvessel density and expression apoptosis-related molecules in colon carcinoma |
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FU Quanhang
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Dongyang People's Hospital
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| Abstract: |
| Objective To investigate the effect of preoperative radio-frequency thermotherapy combined with FOLFOX adjuvant treatment on microvessel density (MVD) and expression of apoptosis- related molecules in colon carcinoma. Methods One hundred and twenty patients with colon cancer treated in our hospital from 2013 January to 2014 June were enrolled and randomly divided into two groups. Patients in study group received preoperative radio-frequency thermotherapy combined with FOLFOX chemotherapy before surgery, those in control group received FOLFOX chemotherapy only. Microvessel density of tumor tissue were assayed by immunohistochemical staining, the mRNA and protein expressions of apoptosis-related molecules in tumor tissue were assayed by Real-time PCR and Western blot respectively. Results MVD in study group was
lower than that in control group(P＜0.05). The mRNA and protein expressions of pro-apoptotic molecules Caspase-3, p16 and
Bax in tumor tissue of study group were higher than those of control group (P＜0.05). The mRNA and protein expression of anti-apoptotic molecules p53, CDC25A and Bcl-2 in tumor tissue of study group were lower than those of control group (P＜ 0.05). Conclusion Preoperative radio-frequency thermotherapy combined with FOLFOX adjuvant treatment may reduce microvessel density, up-regulate pro-apoptotic molecules expression, and down-regulate anti-apoptotic molecules expression in colon cancer. |
| Key words: Colon cancer Radio-frequency thermotherapy Microvessel density Apoptosis Apoptosis gene |
