| 摘要: |
| 目的观察细胞因子诱导的杀伤细胞（DC-CIK）过继免疫治疗前后非小细胞肺癌（NSCLC）患者外周血单个核细胞（PBMC）Toll样受体4（TLR4）mRNA表达变化,及脂多糖刺激下PBMC分泌细胞因子的变化,探讨TLR4在DC-CIK过继免疫治疗中的作用。方法对50例采用DC-CIK治疗的NSCLC患者，运用RT-PCR检测治疗前后PBMCTLR4mRNA表达变化，ELISA法检测治疗前后NSCLC患者PBMC在脂多糖刺激下细胞因子（IL-6、IL-8及TNF-α）分泌的变化。结果DC-CIK治疗后NSCLC患者PBMCTLR4mRNA的表达水平较治疗前显著降低（t=6.272，P＜0.01）；脂多糖刺激下PBMC分泌IL-6（t=17.407，P＜0.01）、IL-8（t=15.244，P＜0.01）及TNF-α（t=12.428，P＜0.01）显著减少。结论DC-CIK过继免疫治疗可能通过降低PBMCTLR4mRNA的表达，提高机体抗肿瘤免疫功能。 |
| 关键词: 树突状细胞 细胞因子诱导的杀伤细胞 非小细胞肺癌 TLR4 |
| DOI：10.12056/j.issn.1006-2785.2017.39.7.2015-1401 |
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| 基金项目: |
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| Changes of TLR4 mRNA expression in peripheral blood of patients with non-small cell lung cancer after DC-CIK immunotherapy |
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WANG Xiyong, YUAN Linna, ZHANG Zhihao
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Jiaxing Armed Police Corps Hosptial
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| Abstract: |
| Objective To investigate the changes of TLR4 mRNA expression in peripheral blood mononuclear cell (PBMC) of patients with non-small cell lung cancer(NSCLC) after DC-CIK immunotherapy. Methods The mRNA expression of TLR4 in PBMC was detected by reverse transcription PCR (PT-PCR) in 50 patients with NSCLC before and after DC-CIK
therapy. The secretion of cytokines (IL-6, IL-8 and TNF-α) of PBMC stimulated with LPS was detected by ELISA before and
after DC-CIK therapy. Results After DC-CIK treatment, the relative expression level of TLR4 mRNA in PBMC of NSCLC patients was significantly decreased(t=6.272, P＜0.01), the secretion of IL-6(t=17.407, P＜0.01), IL-8 (t=15.244, P＜0.01) and TNF-α (t=12.428, P＜0.01) by PBMC stimulated with LPS was significantly decreased. Conclusion DC-CIK immunotherapy can down-regulate TLR4 expression in PBMC of NSCL patients to enhance the anti-tumor immunity. |
| Key words: Dendritic cells(DC) Cytokine-induced killer(CIK) Non-small cell lung cancer TLR4 |
