| 摘要: |
| 目的 观测酪酸梭菌是否对阿司匹林导致的胃溃疡具有预防效应，并对其机制进行初步探讨。方法 取30只健康
雄性ICR 小鼠，随机分成3 组，每组各10 只，即模型组、酪酸梭菌组、奥美拉唑组。酪酸梭菌组以6×108cfu/ 只的菌量，模型组和奥美拉唑组均予以相同体积的无菌培养基，灌胃4d 后再饥饿处理48h，然后以250mg/kg 剂量的阿司匹林灌胃造模，奥美拉唑组小鼠于造模前30min，腹腔注射奥美拉唑（13mg/kg）。造模4h 后，取胃组织分别测定SOD、CAT 活力、MDA 含量；进行常规HE 染色观察胃黏膜组织结构变化，PAS染色判断黏液量的变化，免疫组化法测定Bcl-2、Bax 表达水平。结果 模型组的SOD、CAT 活性明显降低，MDA 含量显著增加，而酪酸梭菌预处理后逆转了该趋势（P＜0.01），与奥美拉唑组比较无统计学差异。HE染色观察，酪酸梭菌能减轻胃黏膜的病理损伤，与奥美拉唑组无统计学差异。PAS 结果提示酪酸梭菌能够增加黏液量。酪酸梭菌组的Bcl-2 表达较模型组显著增多，同时Bax 表达显著减少。结论 酪酸梭菌预处理通过抗氧化应激、增加胃黏液量、上调Bcl-2/Bax 表达比例，减轻阿司匹
林造成的胃溃疡损伤。 |
| 关键词: 酪酸梭菌 阿司匹林 胃溃疡 氧化应激 |
| DOI： |
| 分类号: |
| 基金项目:温州市科技局项目；浙江省新苗计划项目 |
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| Clostridium butyricum prevents gastric ulcer induced by aspirin in mice |
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SHEN Shurong,ZHU Jing,ZHANG Hao,ZENG Jingjing,FU Yaoyang,WANG Yaqi,WANG Fangyan
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Wenzhou Hospital of Intergrated Chinese and Western Medicine
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| Abstract: |
| Objective To investigate the effect of Clostridiumbutyricum (C. butyricum) in prevention of gastric ulcer (GU) induced by aspirin in mice. Methods Thirty ICR mice were randomly divided into model group, bacteria group andomeprazole group (n=10/group). After starvation for 48h, mice were given orally with 250mg/kg aspirin to establish GU model.Mice in bacteria group were orally pretreated with C. butyricum 6×108cfu for 4d before starvation, while mice in model group and omeprazole
groupwere given the samevolume of sterile culture medium.Mice in omeprazole group were intraperitoneally injected with 13mg/kg omeprazole 30 min priorto aspirin treatment. Modeling for 4 h, gastric tissue was to harvest for measuring the activity of SOD and CAT, the content ofMDA, Bcl-2 and Bax, HE staining and PAS staining were performed. Results In model group, the activity of
SOD, CAT were significantly reduced while the content of MDA was significantly increased. The activity of SOD,CAT were increased while the content of MDA was reduced in bacteria group (P＜0.01), there was no significant difference between bacteria and omeprazole group. The Bcl-2 expression increased and Bax expression reduced in bacteria group. PAS stainning showed that the mucus secretion was increased in bacteria group and HE staining showed that the mucosa injury was attenuated in bacteria gorup. Conclusion C. butyricumcan effectively protect the gastric mucosa against damages caused by aspirin in mice. |
| Key words: Clostridiumbutyricum Aspirin Gastric ulcer Oxidative stress |
