| 摘要: |
| 目的探讨黄酒多酚（YWPC）对同型半胱氨酸（Hcy）诱导的大鼠血管平滑肌细胞（VSMCs）基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）表达和活性的影响及其信号通路。方法SD大鼠主动脉VSMCs原代细胞培养鉴定，取4~7代细胞用于实验。YWPC组VSMCs分别用1、10、100mg/L的YWPC干预，采用Westernblot法检测VSMCs中MMP-2、MMP-9、AKT、pAKT的表达情况；明胶酶谱检测VSMCs中MMP-2和MMP-9的活性。用IGF-1（3ng/ml）干预VSMCs8h激活PI3K/AKT通路后，用YWPC干预VSMCs，检测MMP-2、MMP-9的表达和活性。结果Westernblot结果显示，与Hcy组比较，YWPC组VSMCs中MMP-2/9的表达减少，pAKT表达减少（均P＜0.01）；明胶酶谱结果显示，与Hcy组比较，YWPC组VSMCs中MMP-2/9的活性降低。在加入IGF-1后,与对照组比较，IGF-1组VSMCs中pAKT、MMP-2/9的表达和活性增加（P＜0.01）；与YWPC组比较，YWPC+IGF-1组VSMCs中pAKT、MMP-2/9的表达和活性增加（P＜0.01）。结论YWPC通过抑制PI3K/AKT信号通路抑制Hcy诱导的VSMCs中MMP-2/9的表达和活性。 |
| 关键词: 血管平滑肌细胞 黄酒多酚 PI3K/AKT 基质金属蛋白酶 |
| DOI： |
| 分类号: |
| 基金项目:浙江省自然科学基金项目 |
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| Yellow wine polyphenol compounds inhibit homocysteine-induced expression of MMP-2/9 in vascular smooth muscle cells through inhibiting PI3K/AKT pathway |
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LIU Longbin, MENG Liping, JI Zheng, XU Fukang, CHI Jufang, GUO Hangyuan
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Shaoxing People's Hospital
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| Abstract: |
| Objective To investigate the effect of yellow wine polyphenol compounds(YWPC) on expression of MMP-2/9 in rat aortic vascular smooth muscle cells (VSMCs) induced by homocysteine (Hcy) and its mechanism. Methods The primarily cultured VSMCs were incubated with 500滋mol/L Hcy for 12h; then VSMCs were treated with YWPCs in concentration of 1mg/L, 10mg/L or 100mg/L, respectively. IGF-1 (3ng/ml) was used to activate PI3K/AKT pathway. The expressions of MMP-2, MMP-9, AKT, pAKT in VSMCs were detected with Western blotting; the activity of MMP-2/9 was examined with gelatin zymography. Results Western blotting showed that compared with Hcy group, the expressions of MMP-2/9, pAKT in YWPC groups were decreased. Gelatin zymography demonstrated that compared with Hcy group, the activity of MMP-2/9 was decreased in the YWPC groups. Compared with control group, the expressions of pAKT, MMP-2/9 was increased in the IGF-1 group (P＜0.01). Compared with YWPC groups the expression of pAKT, MMP-2/9 was increased in YWPC+IGF-1 group (P＜0.01). Conclusion YWPC inhibits Hcy-induced expression of MMP-2/9 in VSMCs by suppressing PI3K/AKT signaling pathway. |
| Key words: Vascular smooth muscle cells Yellow wne polyphenol PI3K/AKT Matrix Metalloproteinase |
