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miR-29a在系统性红斑狼疮患者血浆中的表达及意义
徐玲娟1, 叶璐璐2, 陈阿琼3, 黄茜茜2, 章慧娣4, 陈朝生4, 薛向阳2
1.浙江中医药大学附属金华市中医医院检验科;2.温州医科大学微生物学和免疫学教研室，分子病毒与疫苗研究所，热带医学研究所;3.宁波李惠利医院风湿免疫科;4.温州医科大学附属第一医院肾内科
摘要:
目的 分析microRNA-29a（miR-29a）在系统性红斑狼疮（SLE）患者血浆中的表达水平及与SLE临床特征的相关性。方法建立检测miR-29a的实时荧光定量PCR（RT-qPCR）方法，分析48例SLE患者和20例健康体检者血浆中miR-29a的表达水平，ROC曲线评估其作为SLE诊断标记物的意义，统计分析其表达与SLE患者临床特征的相关性。结果与健康体检者比较，SLE患者血浆miR-29a表达水平明显上调（Z=3.476，P=0.0009）；血清miR-29a诊断SLE的ROCAUC为0.794（95%CI:0.689~0.898），具较好诊断SLE的效能；抗β2GP1抗体＞20RU/ml患者的血浆miR-29a水平明显升高（P=0.033），Pearson相关分析显示，SLE患者血浆miR-29a表达水平与抗β2GP1抗体水平呈正相关（r=0.584，P=0.001），而与不同SLEDAI评分及其他SLE活动相关指标等差异均无统计学意义（均P＞0.05）。结论miR-29a在SLE患者血浆中的表达水平明显上调，且血浆miR-29a上调与SLE患者自身抗体水平相关，其调节机制尚待进一步的研究。
关键词:  系统性红斑狼疮 临床诊断 微小 RNA miR-29a
DOI：
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基金项目:浙江省科技厅科研基金资助项目；浙江省大学生新苗计划；金华市科技局科研基金资助项目
Expression of miR-29a in plasma of patients with systemic lupus erythematosus and its clinical significance
XU Lingjuan,YE Lulu,CHEN Aqiong,HUANG Qianqian,ZHANG Huidi,CHEN Chaosheng,XUE Xiangyang
Zhejiang Chinese Medical University
Abstract:
Objective To investigate the expression of microRNA-29a (miR-29a) in plasma of patients with systemic lupus erythematosus(SLE) and its clinical significance. Methods The expression of plasma miR-29a was detected with real-time quantitative polymerase chain reaction(qRT-PCR）in 48 SLE patients and 20 healthy subjects. The value of miR-29a for diagnosis of SLE was assessed by the receiver operating characteristic (ROC) curve. The relationship between the plasma miR-29a levels and clinical characteristics was analyzed. Results Compared with the healthy controls, the expression of miR-29a in plasma of patients with SLE was significantly higher (Z=3.476, P=0.0009). The area under the curve (AUC) of ROC was 0.794 (95% CI: 0.689-0.898) . Patients with positive anti-β2GP1 antibody showed higher miR-29a levels in plasma than those with negative anti-β2GP1 antibody (P =0.033). In addition, Pearson analysis indicated that the plasma level of miR-29a was positively correlated with the level of anti-β2GP1 antibodies (r=0.584, P=0.001). However, the SLEDAI score and other clinical indicators of SLE activation were not significantly correlated with the plasma level of miR-29a. Conclusion The expression of miR-29a in the plasma of SLE patients is up-regulated, which were associated with the levels of several auto-antibodies.
Key words:  Systemic lupus erythematosus Clinical diagnosis MicroRNA MiR-29a