| 摘要: |
| 目的 分析并比较人乳头瘤病毒（HPV）E6/E7mRNA和高危型HPV（HR-HPV）DNA检测在无明确诊断意义的不典型鳞状细胞（ASC-US）患者中的临床应用价值。方法 对160例薄层液基细胞学检查（TCT）诊断为ASC-US的患者进行HPVE6/E7mRNA和HR-HPVDNA的检测，并进行阴道镜检查和宫颈活检，结合病理学诊断结果进行统计学分析。结果（1）21～29岁年龄组HR-HPVDNA阳性率明显高于其他年龄组,差异有统计学意义（P＜0.05）；各年龄组HPVE6/E7mRNA的阳性率无统计学差异（P＞0.05）。（2）宫颈上皮内瘤变（CIN）II+级和CINIII+级的HPVE6/E7mRNA阳性率明显高于CINII－级，差异有统计学意义（P＜0.05）；CINII+级和CINIII+级的HR-HPVDNA阳性率亦明显高于CINII-级，差异有统计学意义（P＜0.05）；CINII+级和CINIII+级的HPVE6/E7mRNA阳性率和HR-HPVDNA阳性率均无统计学差异（均P＞0.05）。（3）HPVE6/E7mRNA检测诊断CINII+级的灵敏度为69.2%，低于HR-HPVDNA检测的80.8%，但差异无统计学意义（P＞0.05）；特异度为73.9%，明显高于HR-HPVDNA检测的61.9%，差异有统计学意义（P＜0.05）；根据ROC曲线可见HPVE6/E7mRNA检测诊断CINII+级的效能高于HR-HPVDNA检测。结论与HR-HPVDNA检测相比，HPVE6/E7mRNA检测用于ASC-US患者分流时是否需要进一步作阴道镜检查的判断更为确切。 |
| 关键词: 人乳头瘤病毒 E6/E7mRNA 无明确诊断意义的不典型鳞状细胞 |
| DOI： |
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| Application of HPV E6/E7 mRNA and high-risk HPV DNA tests in patients with ASC-US |
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PAN Qiangwei,CHEN Dake,CHEN Yumei,PAN Jiajia,LV Jieqiang
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the Third Clinical Istituded Affiliated to Wenzhou Medical University，Wenzhou People's hospital
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| Abstract: |
| Objective To evaluate the application of human papillomavirus (HPV) E6/E7mRNA and high-risk HPV
(HR-HPV) DNA tests in patients with atypical squamous cell of undetermined significance (ASC-US). Methods One hun- dred and sixty patients with ASC-US cytology underwent HPVE6/E7 mRNA and HR-HPV DNA tests, colposcopy/biopsy. The clinical value of two tests was analyzed and compared. Results The positive rate of HR-HPV DNA in age group 21~29y was significantly higher than those in other age groups(P＜0.05), but no significant difference in positive rate of HPVE6/E7mRNA was found among different age groups (P ＞0.05). The positive rate of HPV E6/E7 mRNA and HR-HPV DNA for CINII+ and CINIII+ were significantly higher than that of CINI or normal subjects (P＜0.05). No significant differences were found in positive rate of HPV E6/E7 mRNA and HR-HPV DNA between CINII+ and CINIII+ groups (P ＞0.05). The sensitivity of HPV E6/E7mRNA and HR-HPV DNA for CINII+ was 69.2% and 80.8%, respectively(P ＞0.05); the specificity was 73.9% and 61.9%, respectively(P＜
0.05). According to ROC curve, the diagnostic power of HPVE6/E7mRNA test for CIN II+ was higher than that of HR-HPV DNA. Conclusion Compared to HR-HPV DNA test, HPVE6/E7mRNA test is more effective for colposcopy referral in triage of patients with ASC-US. |
| Key words: Human papillomavirus E6/E7mRNA Atypical squamous cell of undetermined significance |
