| 摘要: |
| 目的探索子宫内膜异位症（EMs）中miR-143、miR-145和miR-126的差异表达，以及不同月经周期的影响。方法利用realtimeRT-PCR技术，比较miR-143、miR-145和miR-126在EMs患者在位内膜（EU，2例）、异位内膜（EC，14例）、配对EU+EC（14例），与非EMs患者内膜（EN，28例）中的表达差异；并分析EN和EC组不同月经周期对miR-143、miR-145和miR-126表达的影响。结果不同月经周期的影响分析，发现仅EN标本中miR-143增生期表达量高于分泌期，差异有统计学意义（P＜0.05）。与EN相比，miR-143和miR-145在EU中均表达上调（P＜0.05），miR-143、miR-145和miR-126在EC中均表达上调（P＜0.01）。配对EU-EC中，miR-143、miR-145和miR-126在EC中均表达上调（P＜0.01）。结论miR-143、miR-145和miR-126在EMs中表达高于非EMs，在EC中表达高于EU。 |
| 关键词: 子宫内膜异位症 miR-143 miR-145 miR-126 |
| DOI： |
| 分类号: |
| 基金项目:浙江省卫生厅平台重点资助项目；温州市科技局课题 |
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| Differential expression of miR-143, miR-145 and miR-126 in endometriosis |
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Zheng Bingbing,Xu Chaoyi,Zhao Yiping,Han Lingzhi,Yu Louchao,Duan Ping
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the First Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the differential expression of miR-143,miR-145 and miR-126 in endometriosis(EMs). Methods The expressions of miR-143, miR-145 and miR-126 were detected with real-time RT-PCR in eutopic endometrium (EU, n=2), ectopic endometrium(EC, n=14), paired EU+EC (n=14) from women with EMs and normal endometrium(EN, n=28) from women without EMs. The expressions of miR-143,miR-145 and miR-126 were also detected in different menstrual cycle of EN and/or EC. Results Compared to EN, the expression of miR-143, miR-145 in EU was up-regulated (P＜0.05). Compared to EN, the expression of miR-143,miR-145 and miR-126 in EC was up-regulated (P＜0.01). Compared to corresponding EU samples, the expression of miR-143,miR-145 and miR-126 in EC was up-regulated (P＜0.01). The miR-143 in proliferative endometrium was up-regulated, compared to secretory endometrium in EN group (P＜0.05). Conclusion In endometriosis the expressions of miR-143, miR-145 and miR-126 in ectopic endometrium are up-regulated. |
| Key words: Endometriosis MiR-143 MiR-145 MiR-126 |
