| 摘要: |
| 目的 探讨基质窖蛋白1（Cav-1）、乳酸脱氢酶B（LDH-B）和丙酮酸激酶2（PKM2）在人肺腺癌（PAC）组织中的表达并分析3者的相关性及其临床意义。方法 采用量子点免疫荧光组织化学（QDs-IHC）技术检测68例PAC组织和16例非癌性肺组织中基质Cav-1、LDH-B和PKM2的表达情况。结果基质Cav-1在PAC组织的阳性率为58.8%，与非癌变肺组织的阳性率100%相比，差异有统计学意义（P＜0.05）。肺腺癌组织中LDH-B和PKM2的阳性率分别为76.5%、70.6%，均高于非癌性肺组织（均P＜0.05）。PAC中、低分化组PKM2的阳性率均高于高分化组（均P＜0.05）。结论基质Cav-1缺失，LDH-B、PKM2的高表达可能促进PAC的恶性进展。 |
| 关键词: 肺腺癌 窖蛋白 1 乳酸脱氢酶 B 丙酮酸激酶 2 肿瘤能量代谢 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目 |
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| Expression of stromal caveolin-1, LDH-B and PKM2 in pulmonary adenocarcinoma |
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CHEN Fuchun,PAN Qi,FU Pin,LU Funian,CHEN Honglei
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Traditional Chinese Medical Hospital of Wenling
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| Abstract: |
| Objective To investigate the expression of stromal caveolin-1 (Cav-1), L-lactate dehydrogenase B (LDH-B) and pyruvate kinase M2 (PKM2) in pulmonary adenocarcinoma (PAC) tissues. Methods The expression of stromal Cav-1, LDH-B and PKM2 proteins in 68 specimens of PAC and 16 specimens of noncancerous lung tissue with quantum dots immunofluorescent histochemistry(QDs-IHC), Results The positive rates of stromal Cav-1 in PAC tissues and non-cancerous lung tissues were 58.8％ and 100.0%(P＜0.05). The positive rates of LDH-B and PKM2 expression in PAC tissues were 76.5％ and 70.6％, respectively, which were higher than those in noncancerous lung tissues(P＜0.05). The expression of PKM2 in mid-
dle/low-differentiated PAC was higher than that in well-differentiated PAC(P＜0.05). Conclusion The results indicated that stro- mal Cav-1 is down-regulated and LDH-B and PKM2 are up-regulated in pulmonary adenocarcinoma. |
| Key words: Pulmonary adenocarcinoma Caveolin-1 L-lactate dehydrogenase B Pyruvate kinase M2 Tumor en-
ergy metabolism |
