| 摘要: |
| 目的探讨双特异性磷酸酶1（DUSP1）在涎腺腺样囊性癌（SACC）组织中的表达及其意义。方法采用免疫组化、qRT-PCR和Westernblot检测48例SACC患者的癌组织和20例癌旁正常组织中DUSP1的蛋白和基因表达水平，并收集相关临床资料，分析DUSP1蛋白表达与SACC患者临床病理特征间的关系。结果48例SACC患者标本中18例（37.5%）DUSP1阳性表达，30例（62.5%）阴性表达。qRT-PCR结果显示，SACC癌组织中DUSP1的基因表达阳性率显著低于癌旁正常组织（P＜0.05），Westernblot结果与其一致。不同T分期、淋巴结转移、神经侵犯、局部侵犯的患者间比较，DUSP1蛋白表达阳性率均有统计学差异（均P＜0.05），而不同性别、年龄、肿瘤部位、组织学类型、远处转移的患者间比较，DUSP1蛋白表达阳性率均无统计学差异（均P＞0.05）。结论DUSP1在SACC中表达下降，与T分期、淋巴结转移、神经侵犯、局部侵犯有关，有望成为SACC预后的潜在标志物之一。 |
| 关键词: 双特异性磷酸酶 1 涎腺腺样囊性癌 预后 |
| DOI： |
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| 基金项目:浙江省自然科学基金浙江省医药卫生科技项目平台计划学科带头人项目 |
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| Expression of DUSP1 in salivary adenoid cystic carcinoma and its clinicopathological significance |
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CAO Jun,LING Zhiqiang,XIA Minghua,ZHENG Chuanming
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the Second Clinical Medical College，Zhejiang Chinese Medical University
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| Abstract: |
| Objective To investigate the expression of dual specificity phosphatase1 (DUSP1) in salivary adenoid cystic carcinoma (SACC) and its clinicopathological significance. Methods Immunohistochemisty, real-time fluorescence quantitative PCR (RT-qPCR) and Western blot method were used to determine the expression of DUSP1 in 48 samples of SACC tissue and 20 samples of pericancerous tissue. The relationship between DUSP1 expression and clinicopathological features was analyzed. Results Immunohistochemistry showed that in 48 salivary gland ACC samples, 18 cases (37.5%) were DUSP1-positive, while 30 (62.5%) were DUSP1-negative．qRT-PCR result revealed that the expression of DUSP1 was significantly lower in SACC tissues compared to pericancerous tissues (P ＜0.05), Western blot result was in accordance with qRT-PCR. There were significant differences in expression of DUSP1 among patients with different T stages, lymph node metastasis, nerve invasion and local invasion (P＜0.05), but no significant differences among patients with different gender, age, tumor location, histological types and
distant metastasis (P ＞0.05). Conclusion The expression of DUSP1 is correlated with T stage, lymph node metastasis, nerve in- vasion and local invasion in SACC, indicating that it may be used asa potential negative prognostic indicator for SACC. |
| Key words: Dual specificity phosphatase1 Salivary adenoid cystic carcinoma Prognosis |
