| 摘要: |
| 目的探索甲状旁腺激素（PTH）和骨硬化蛋白抗体（Scl-Ab）联合治疗雌性SD大鼠骨质疏松症的疗效，为临床应用提供实验基础。方法从70只健康雌性SD大鼠随机选取65只行双侧卵巢切除术，其余5只行假手术；术后在正常环境下饲养12周，从双侧卵巢切除大鼠中随机选取5只检测胫骨骨密度，较假手术大鼠下降37.64%，证实骨质疏松大鼠模型建立成功。遂将余下60只双侧卵巢切除大鼠随机分成4组：未用药组15只（其中2只大鼠因伤口感染而死亡）、Scl-Ab组15只（Scl-Ab25mg/kg皮下注射，2次/周）、PTH组15只（PTH60滋g/kg皮下注射，3次/周）、PTH+Scl-Ab组15只（Scl-Ab25mg/kg皮下注射，2次/周；同时PTH60滋g/kg皮下注射，3次/周）。治疗12周后处死大鼠取胫骨行Micro-CT、HE组织切片和骨生物力学检测与比较。结果Micro-CT三维重建分析，药物治疗12周后Scl-Ab组、PTH组和PTH+Scl-Ab组骨小梁数量较未用药组明显增多，且骨小梁更粗、联系更为紧密；胫骨干骺端微观参数改善较未用药组更为明显，差异均有统计学意义（均P＜0.05），其中PTH+Scl-Ab组最为明显（P＜0.05）。HE组织切片可见Scl-Ab组、PTH组和PTH+Scl-Ab组骨小梁之间联系紧密，排列规律，且胫骨干骺端骨量增加，胫骨局部骨组织矿化明显，其中PTH+Scl-Ab组最为明显。骨生物力学检测结果显示Scl-Ab组、PTH组和PTH+Scl-Ab组最大载荷、刚度值均高于未用药组（均P＜0.05），其中PTH+Scl-Ab组亦分别高于Scl-Ab组和PTH组（均P＜0.05）。结论PTH、Scl-Ab治疗雌性SD大鼠骨质疏松症的疗效明显，其中两药联用疗效更佳。 |
| 关键词: 去势大鼠 骨质疏松症 甲状旁腺激素 骨硬化蛋白抗体 |
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| Effect of combined treatment with parathyroid hormone and sclerostin antibody capsules in the treatment of osteoporosis in female SD rats |
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CHEN Xin, PAN Yuehao, JIN Guohua, LOU Shuchang
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Yiwu Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the efficacy of parathyroid hormone (PTH) and sclerostin antibody (Scl-Ab) for treatment of osteoporosis in female SD rats. Methods Female SD rats underwent bilateral ovariectomy (OVX, n=65) or sham-operation (sham, n=5). Five overiectomized rats were randomly selected and bone mass density (BMD) of tibia was measured to approve the success of osteoporosis model. The remaining overiectomized rats were randomly divided into 4 groups with 15 in each group. PTH(60滋g/kg, 3/wk), Scl-Ab (25mg/kg, 2/wk) and PTH (60滋g/kg, 3/wk) plus Scl-Ab (25mg/kg, 2/wk) were injected subcutaneously for 12 wks to rats in PTH, Scl-Ab and PTH+Scl-Ab groups respectively, no drugs were given to rats in OVX group. The tibial metaphyseals of rats were harvested for evaluation. The results of treatment for osteoporosis were evaluated by micro-computerized tomography (micro-CT), histological examination and biomechanical measurement. Results Comparison with OVX group, the tibial metaphyseals from groups Scl-Ab, PTH and PTH+Scl-Ab showed a higher BMD, BV/TV, Tb Th, Tb N, Conn D, maximum load, stiffness and a lower Tb Sp, and the effects were strongest in PTH+Scl-Ab group. The effect of Scl-Ab or PTH alone was significantly lower than that of combined use in prevention and treatment of osteoporosis of ovariectomized rats. Conclusion The results indicate that Scl-Ab plus PTH had an additive effect on osteoporosis in overiectomized rat. |
| Key words: OVX rats Parathyroid hormone Osteoporosis Sclerostin antibody |
