| 摘要: |
| 目的探讨单核细胞趋化蛋白-1（MCP-1）基因多态性与浙江局部地区汉族人群2型糖尿病（T2DM）的相关性。方法采用病例对照研究，选取浙江局部地区汉族人群中T2DM患者208例（T2DM组）和健康体检者209例（对照组），采用聚合酶链反应-限制性片段长度多态性方法检测受试者CCL2rs1024611（-2518A/G）的基因型，并用DNA测序法鉴定部分结果。结果CCL2rs1024611基因多态性与T2DM发生相关，T2DM组和对照组G基因频率差异有统计学意义（P＜0.01），T2DM组和对照组GG基因型频率差异有统计学意义（P＜0.01），多因素logistic回归分析显示，GG基因型频率可显著降低T2DM风险（校正后OR=0.201，95%CI：0.077~0.526，P＜0.01）。结论CCL2rs1024611基因多态性与T2DM发生相关，其中GG基因型可能是T2DM的保护性因素，G等位基因可能降低汉族浙江局部地区汉族人群T2DM的风险。 |
| 关键词: 单核细胞趋化蛋白 -1 2 型糖尿病 单核苷酸多态性 |
| DOI： |
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| 基金项目: |
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| Association of monocyte chemoattractant protein -1 gene polymorphism with risk of type 2 diabetes mellitus in Han population in Zhejiang province |
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MA Jiangbo, XU Xin, MA Gaigai, SHEN Jie, HUANG Qinhua, SHI Yuping
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Zhejiang provincial People's Hospital
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| Abstract: |
| Objective To investigate the association of monocyte chemoattractant protein-1 (MCP-1) gene rs1024611 (-2518A/G) polymorphisms with risk of type 2 diabetes mellitus (T2DM) in Han population of Zhejiang province. Methods A total of 208 patients with T2DM and 209 healthy control subjects in Han population of Zhejiang province were enrolled in this study. Genotyping was performed by polymerase chain reaction-based restriction fragment length polymorphism and subsequently confirmed by DNA sequencing. Odds ratio(OR) and 95% confidence intervals(CI) were calculated for the risk of genotypes and alleles. Results MCP-1 gene polymorphism rs1024611 (-2518A/G) was conformed to the Hardy-Weinberg equilibrium. The statistical differences in the G allele (43.7% vs.55.7%, P＜0.01) and GG genotype (19.2% vs.33.5%, P＜0.01)
were found between T2DM patients and controls. The GG genotype was associated with a decreased risk of T2DM(OR=0.201, 95% CI: 0.077-0.526,P＜0.01). Conclusion The MCP-1 gene rs1024611(-2518A/G) may be associated with T2DM suscep- tibility in Han population of Zhejiang province; GG genotype may be the protect factor for T2DM and G allele may reduce the risk of T2DM. |
| Key words: Monocyte chemoattractant protein-1 Type 2 diabetes mellitus Single nucleotide polymorphism |
