| 摘要: |
| 目的探寻聚维酮碘溶液快速灭活子宫内膜细胞的最佳有效浓度和作用时间，并对聚维酮碘溶液诱导子宫内膜细胞凋亡作用进行研究。方法不同浓度（0.1%~2.0%）、不同时间（15~90s）的聚维酮碘溶液作用原代培养子宫内膜细胞，采用MTT染色实验检测聚维酮碘溶液对子宫内膜细胞的灭活作用，Hoechst33342染色实验和流式细胞术检测聚维酮碘溶液对子宫内膜细胞促凋亡作用。结果聚维酮碘溶液对子宫内膜细胞具有明显的灭活作用，呈浓度依赖和时间依赖，且随着聚维酮碘溶液浓度的增加，子宫内膜细胞凋亡率明显升高（P＜0.05）。结论聚维酮碘溶液可有效灭活子宫内膜细胞，对预防腹壁子宫内膜异位症的发生有着重要的临床应用意义。 |
| 关键词: 聚维酮碘 腹壁子宫内膜异位症 子宫内膜细胞 凋亡 |
| DOI： |
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| 基金项目:浙江省医药卫生科技项目(2014KYB033) |
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| Povidone-iodine solution inactivates human endometrial cells |
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ZHOU Huifang, HONG Min, WANG Mengtian, SHAN Jiawen, WU Jianyu, YANG Liwei, HE Xujun
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Zhejiang provincial People's Hospital
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| Abstract: |
| Objective To investigate the effect of povidone-iodine at different concentration on the growth of human endometrial cells. Methods Primary cultured endometrial cells were treated with different concentrations (0.1% -2.0% ) of povidone-iodine at different time (15-90s), respectively. The viability, morphological changes, and apoptosis of endometrial cells were assessed by MTT assay, Hoechst33342 staining, and Annexin V/PI flow cytometry, respectively. Results The results indicated that povidone-iodine remarkably inhibited endometrial cells growth at a dose-dependent and time-dependent manner (P＜0.05). Conclusion Povidone-iodine solution can effectively inactivate endometrial cells, which may potential clinical use in prevention of endometriosis. |
| Key words: Povidone-iodine Abdominal wall endometriosis Endometrial cells Apoptosis |
