| 摘要: |
| 目的探讨Toll样受体10（TLR10）基因多态性位点Rs4129009（Ile775Val）与慢性HBV感染的关系。方法采集428例慢性HBV感染患者、210例健康对照人群外周血并提取全基因组DNA。采用聚合酶链反应（PCR）法扩增特定基因多态性位点，产物经纯化后直接测序分析。结果两组均符合Hardy-Weinberg平衡（均P＞0.05），即研究对象具有群体代表性。与健康对照组比较，HBV感染组变异型（AG+GG）、等位基因G频率均较低（均P＜0.01）。高病毒载量（＞106IU/ml）、HBeAg阳性的变异型基因比例较高（均P＜0.01），而HBV感染程度、乙肝家族史与基因多态性无关（均P＞0.05）。结论TLR10基因Rs4129009可能与慢性HBV感染相关。 |
| 关键词: Toll 样受体 10 基因多态性 乙型肝炎病毒 |
| DOI：10.12056/j.issn.1006-2785.2017.39.13.2016-1352 |
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| Association of TLR10 Rs4129009 gene polymorphism with chronic hepatitis B virus infection |
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TANG Yongzhi, CHEN Qiaoling, JIANG Ting, ZHU Min, YAN Fei, CHEN Huazhong, ZHU Jiansheng
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The Affiliated Taizhou Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the relationship between the gene polymorphism of Toll like receptor 10 (TLR10) and chronic HBV infection. Methods The peripheral blood samples from 428 chronic HBV infected patients and 210 healthy controls were collected. The extracted whole genome DNA was specifically amplified by polymerase chain reaction (PCR). Then, direct sequencing was performed. Results The characteristics of two groups were in accordence with Hardy-Weinberg balance (both P ＞0.05). The frequency of AG+GG genotypes and G allele in HBV infected group were lower than those in healthy control (P＜0.01). The subgroup analysis demonstrated that variant gene in HBVDNA ＞ 106IU/ml group and HBeAg positive group were higher than the other groups (P＜0.01), nevertheless, no significant difference were found in the subgroups of the severity of HBV
infection and family medical history (P ＞0.05). Conclusion Polimorphism of Rs4129009 in TLR10 may be related to chronic HBV infection. |
| Key words: Toll like receptor 10 Polymorphism Hepatitis B virus |
