| 摘要: |
| 目的探讨脑胶质瘤组织中PER1、PER2、CRY1、CRY2、CLOCK、BMAL1和NPAS2时钟基因启动子区甲基化状态与脑胶质瘤发生、发展的关系。方法采用甲基化特异性PCR检测脑胶质瘤组织及其癌旁正常组织（对照）PER1、CRY1、CRY2、CLOCK、BMAL1、NPAS2等时钟基因启动子区甲基化状态。结果脑胶质瘤组织与癌旁正常组织中PER1、CLOCK时钟基因启动子区均未有甲基化；脑胶质瘤组织中NPAS2、PER2时钟基因启动子区甲基化频率明显高于癌旁正常组织（均P＜0.05）；两种组织中CRY1、CRY2和BMAL1时钟基因启动子区甲基化频率比较，差异均无统计学意义（均P＞0.05）。不同级别脑胶质瘤组织中PER1、CLOCK时钟基因启动子区均未有甲基化；高级别脑胶质瘤（Ⅲ～Ⅳ级）组织中NPAS2时钟基因启动子区甲基化频率高于低级别胶质瘤（Ⅰ～Ⅱ级）组织（P＜0.05）；不同级别脑胶质瘤组织中CRY1、CRY2、BMAL1和PER2时钟基因启动子区甲基化频率比较，差异均无统计学意义（均P＞0.05）。结论NPAS2、PER2时钟基因启动子区甲基化频率明显增高；NPAS2时钟基因启动子区甲基化频率越高，脑胶质瘤的恶性程度越高。NPAS2、PER2时钟基因启动子区DNA甲基化修饰可能是脑胶质瘤发生、发展的重要机制。 |
| 关键词: 脑胶质瘤 启动子区 甲基化 时钟基因 |
| DOI：10.12056/j.issn.1006-2785.2017.39.18.2016-1656 |
| 分类号: |
| 基金项目:浙江省医药卫生科技计划项目（2014KYA168） |
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| Methylation status of clock gene promoter region in glioma patients |
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Hangzhou First People's Hospital
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| Abstract: |
| Objective To investigate the methylation status of PER1, PER2, CRY1, CRY2, CLOCK, BMAL1 and NPAS2 gene promoter regions in glioma tissues and its clinical significance. Methods The frequencies of methylation of PER1, PER2, CRY1, CRY2, CLOCK, BMAL1 and NPAS2 gene promoters were determined in glioma tissues and in surrounding non-glioma tissues (control group) were detected by methylation-specific PCR method. Results PER1 and CLOCK genes were devoid of
methylation in promoter region. Differences in the promoter methylation frequencies of CRY1, CRY2 and BMAL1 genes between glioma and control group were not significant (P ＞0.05). Promoter methylation frequencies of NPAS2 and PER2 genes were significantly were elevated in glioma tissue compared to control group(P＜0.05), and moreover, promoter methylation frequency of NPAS2 was enhanced with increasing extent of malignancy(P＜0.05). Conclusion DNA-methylation modification in the promoter
of NPAS2 and PER2 genes may be an important mechanism underlying occurrence and development of glioma. |
| Key words: Glioma Promoter Methylation Clock gene |
