| 摘要: |
| 目的探讨高糖腹膜透析液对人腹膜微血管内皮细胞（HPMECs）中GLUT1、SGLT1、TGF-β1、VEGF-A、CTGF表达的影响。方法体外培养HPMECs并分为5组，N组用含葡萄糖的无酚红RPMI-1640培养基培养；P组用2.5%葡萄糖腹膜透析液培养；GN组用含50g/ml葡萄糖转运蛋白1（GLUT1）拮抗剂根皮素的2.5%葡萄糖腹膜透析液培养；SN组用含10g/ml钠-葡萄糖共转运载体1（SGLT1）拮抗剂根皮苷的2.5%葡萄糖腹膜透析液培养；GN+SN组用含50g/ml根皮素和含10g/ml根皮苷的2.5%葡萄糖腹膜透析液培养。分别采用RT-PCR法、Westernblot法检测各组HPMECsGLUT1、SGLT1、细胞因子转化生长因子-β1（TGF-β1）、血管内皮生长因子-A（VEGF-A）、结缔组织生长因子（CTGF）的mRNA、蛋白表达水平。结果5组HPMECsGLUT1、SGLT1、TGF-β1、VEGF-A、CTGF的mRNA、蛋白表达水平比较均有统计学差异（均P＜0.05）。与N组比较，P组HPMECsGLUT1、SGLT1、TGF-β1、VEGF-A、CTGF的mRNA、蛋白表达水平均升高（均P＜0.05）；与P组比较，GN组、SN组、GN+SN组HPMECsGLUT1、SGLT1、TGF-β1、VEGF-A、CTGF的mRNA、蛋白表达水平均降低（均P＜0.05）。结论高糖腹膜透析液或通过上调HPMECs中GLUT1、SGLT1的表达，进而上调TGF-β1、VEGF-A、CTGF的表达，从而起促腹膜纤维化作用。 |
| 关键词: 腹膜微血管内皮细胞 葡萄糖转运蛋白 1 钠 - 葡萄糖共转运载体 1 细胞因子转化生长因子 -β1 血管内皮
生长因子 -A 结缔组织生长因子 |
| DOI：10.12056/j.issn.1006-2785.2017.39.8.2016-1678 |
| 分类号: |
| 基金项目:浙江省自然科学基金资助项目（Y13H050021）；宁波市科技计划项目（2015C50001）；宁波市鄞州区科技计划项目（鄞科[2011]69号-26） |
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| Expression of GLUT1, SGLT1, TGFβ-1, VEGF-A, CTGF in human peritoneal microvascular endothelial cells cultured with high glucose peritoneal dialysis fluid |
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NIE Zhenyu, HONG Mengqi, BAO Beiyan, LI Guofu, CHEN Zhenyue, YU Xiongwei
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Ningbo Vrology and Nephrology Hospital
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| Abstract: |
| Objective To investigate the effect of high glucose-based peritoneal dialysis fluid (PDF) on the expression of glucose transporters1 (GLUT1), Na+/glucose cotransporters1 (SGLT1), transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor-A (VEGF-A) and connective tissue growth factor (CTGF) in human peritoneal microvascular endothelial cells (HPMECs). Methods The cultured HPMECs were divided into 5 groups: normal group (N), peritoneal dialysis (containing 2.5% glucose) group (P); GLUT1 antagonists group(GN), SGLT1 antagonists group (SN); GLUT1 antagonists+ SGLT1 antagonists group (GN+SN). The mRNA and protein expression of GLUT1, SGLT1, TGF-β1, VEGF-A and CTGF in HPMECs were detected by RT-qPCR and Western blotting, respectively. Results There were significant differences in mRNA and protein expression of GLUT1, SGLT1, TGF-β1, VEGF-A and CTGF among 5 groups (P＜0.05). Compared with group N, the mRNA and protein expression of GLUT1, SGLT1, TGF-β1, VEGF-A and CTGF in group P was increased significantly (P＜0.05). Compared with group P, the mRNA and protein expression of GLUT1, SGLT1, TGF-β1, VEGF-A and CTGF in groups GN, SN and GN+SN was decreased significantly (P＜0.05). Conclusion High glucose peritoneal dialysis fluid may up-regulate the expression of GLUT1, SGLT1, which may further induce the up-regulating expression of TGF-β1, VEGF-A and CTGF in human peritoneal microvascular endothelial cells, resulting in the peritoneal fibrosis. |
| Key words: Peritoneal microvascular endothelial cells Glucose transporters1 Na+/glucose cotransporters1 TGF-β1 VEGF-A CTGF |
