| 摘要: |
| 目的探讨丹皮酚（Pae）预处理抑制1-甲基-4-苯基吡啶（MPP+）诱导的BV2小胶质细胞激活的作用及机制。方法以MPP+刺激的BV2小胶质细胞建立帕金森病细胞模型，以不同浓度的Pae预处理BV2小胶质细胞，采用CCK-8、免疫细胞荧光法、ELISA分别检测BV2小胶质细胞增殖活性、吞噬活性和炎症因子（TNF-α、IL-1β、IL-6）的释放。结果与对照组比较，MPP+0.1滋mol/L可明显诱导BV2小胶质细胞的激活，促进BV2小胶质细胞增殖活性、吞噬活性的增强以及炎症因子TNF-α、IL-1β、IL-6的释放（均P＜0.01）。与MPP+组比较，MPP++Pae（5、25滋mol/L）组预处理后MPP+诱导的BV2小胶质细胞增殖活性、吞噬活性明显减弱，BV2小胶质细胞释放的炎症因子IL-1β、TNF-α和IL-6明显减少，差异均有统计学意义（均P＜0.05）。结论MPP+可诱导BV2小胶质细胞激活，促进细胞增殖、吞噬活性增强以及炎症因子释放；经MPP++Pae（5、25滋mol/L）预处理后，可抑制以上效应，推测与抑制小胶质细胞吞噬活性及抗炎作用有关。 |
| 关键词: 丹皮酚 帕金森病 小胶质细胞 炎症因子 |
| DOI：10.12056/j.issn.1006-2785.2017.39.6.2016-1815 |
| 分类号: |
| 基金项目:国家自然科学基金项目（81401566）；浙江省自然科学基金（LQ15H090005）；浙江省医药卫生科技项目（2015KYB076）；浙江省中医药管理局科研基金项目（2015ZB012） |
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| Paeonol pretreatment inhibits MPP+ induced BV2 microglia activation in Parkinson disease |
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WANG Hao, ZHANG Zhenzhong, LI Zhongchun, ZHAO Bing
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Tongde Hospital of Zhejiang Province
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| Abstract: |
| Objective To investigate the effects of paeonol on MPP+ induced BV2 microglia activation, which is a cell model of Parkinson disease. Methods After MPP+ and paeonol pretreatment, cell viability was detected by CCK-8 assay; microglial phagocytosis was assessed by immunostaining methods, and inflammatory cytokine release was determined
according to the ELISA kits. Results MPP+ treatment(0.1滋mol/L) significantly enhanced BV2 microglial cell viability, increased
microglial phagocytosis, and enhanced inflammatory cytokine of TNF-α, IL-1β, IL-6 release. Compared to the MPP+ group, Paeonol treatment (5, 25滋mol/L) significantly inhibited the increase of cell viability, decreased microglial phagocytosis, and reduced TNF-α, IL-1β, IL-6 release. Conclusion Paeonol can prevent MPP+ induced BV2 microglia activation, and the mechanism may be related to inhibit microglial phagocytosis, and the anti-inflammatory effects. |
| Key words: Paeonol Parkinson disease Microglia Inflammatory cytokine |
