| 摘要: |
| 目的探讨miR-181家族在人多形性脑胶质母细胞瘤（GBM）中的表达及其与临床病理特征的关系。方法采用茎环实时荧光定量PCR（RT-qPCR）法检测40例胶质母细胞瘤中miR-181a、miR-181b、miR-181c、miR-181d的表达水平，并分析其与胶质母细胞瘤临床病理特征的关系。采用慢病毒转染上调胶质瘤细胞系U251miR-181a的表达，分析miR-181a对U251克隆形成、细胞增殖和凋亡的影响。结果miR-181a高表达的GBM患者生存预期明显延长（P＜0.05）；且miR-181a表达水平越高，GBM瘤周水肿越明显（P＜0.05）。O6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）阳性组的GBMmiR-181a表达明显高于阴性组（P＜0.05）。上调miR-181a的表达后，U251胶质瘤细胞克隆形成数明显少于对照组（P＜0.05），且细胞生长抑制在第5天最明显，差异有统计学意义（P＜0.01）；对细胞凋亡起促进作用（P＜0.05）。结论miR-181a表达水平与GBM患者的临床预后相关。miR-181a表达减少与GBM的发生密切相关。 |
| 关键词: miR-181 胶质母细胞瘤 MGMT |
| DOI：10.12056/j.issn.1006-2785.2017.39.18.2016-2010 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目（LY16H160053）；浙江省医药卫生科技计划项目（2012RCA042）；温州市科技计划项目（Y20120008） |
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| Expression of miR-181 family in glioblastoma and its clinical significance |
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the First Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the expression of miR-181 family in multiform glioblastoma（GBM）and its correlation with clinicopathological characteristics of the tumor. Methods The expression of miR-181a, miR-181b, miR-181c and miR-181d was detected by loop RT-qPCR in tumor tissue specimens of 40 GBM patients. The relationship between miR-181 expression and clinicopathological characteristics was analyzed. The lentiviral vector carrying miR-181a was transfected into U251 cells, the proliferation clone-forming ability and apoptosis of miR-181a-transfected U251 cells were determined. Results The up-regulation of miR-181a expression was correlated with the survival of patients and peritumoral brain edema (P＜0.05). Expression of miR-181a was significantly higher in patients with O6-methylguanine-DNA methyltransferase(MGMT) positive than those with MGMT negative (P ＜0.05). Cell studies indicated that the over-expression of miR-181a significantly inhibited the proliferation of U251 cells(P＜0.01), reduced clone-forming ability (P＜0.05), and increased apoptosis (P＜0.05). Conclusion The expression of MiR-181a may be associated with the prognosis of GBM, and down-expression of miR-181a may be involved in the development of GBM. |
| Key words: miR-181 Glioblastoma MGMT |
