| 摘要: |
| 目的研究miR-630在人肺癌组织中的表达，探讨miR-630对肺腺癌细胞系（A549）细胞迁移侵袭的影响及其机制。方法收集37例肺腺癌组织标本及癌旁正常肺组织，通过RT-PCR检测各标本中miR-630、SOX4mRNA的表达情况，Pearson相关分析检验miR-630与SOX4的相关性；通过转染miR-630mimic和miR-630NC至离体培养的A549细胞中，Westernblot检测A549细胞中COL1A1、COL1A5、SOX4的表达情况，划痕试验和Transwell法观察细胞迁移侵袭情况，荧光素酶素试验验证SOX4是miR-630的靶基因。结果与癌旁正常肺组织比较，miR-630在肺腺癌组织中的表达降低（P＜0.05）；肺腺癌组织中SOX4mRNA的表达增加（P＜0.01）；miR-630与SOX4mRNA的表达量呈负相关（r=-0.344，P＜0.01）。与对照组比较，转染miR-630mimic后A549细胞的迁移侵袭减少（P＜0.01），细胞中COL1A1、COL1A5、SOX4的表达减少（均P＜0.05）；荧光素酶试验结果显示，miR-630能够降低SOX4-3′-UTR质粒的荧光素活性（P＜0.05）。结论miR-630在肺腺癌组织中低表达；miR-630可能是通过下调SOX4抑制A549细胞的迁移和侵袭。 |
| 关键词: 肺腺癌 微小 RNA-630 SOX4 侵袭 |
| DOI：10.12056/j.issn.1006-2785.2017.39.12.2016-2015 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目（LY14H0200002） |
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| Expression of miR-630 in lung adenocarcinoma and its effect on tumor migration and invasion |
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XIE Mingfang, TANG Mengmeng, DING Wei
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Keqiao District Hospital of Traditional Chinese Medicine
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| Abstract: |
| Objective To investigate the expression of miR-630 in lung adenocarcinoma and its effect on tumor invasion and metastasis. Methods The expressions of miR-630 and SOX4 mRNA were detected by RT-PCR in 37 samples of lung adenocarcinoma tissue and matched normal lung tissue. Pearson correlation analysis was performed to test the correlation of miR-630 with SOX4 mRNA. Human lung adenocarcinoma A549 cells were transfected with miR-630. The migratory ability of A549 cells was tested by wound healing and Transwell assays; the expressions of COL1A1, COL1A5 and SOX4 in A549 cells were detected by Wester n blotting; Luciferase assay was used to confirmed whether SOX4-3'-UTR was the target gene of
miR-630. Results Compared with normal lung tissue, the expression of miR-630 was decreased and SOX4 mRNA was significantly increased in lung adenocarcinoma (P ＜0.05, P ＜0.01). Pearson correlation analysis showed that miR-630 was negatively correlated with SOX4 mRNA (r=-0.344, P ＜0.01). In vitro experiment, compared with the wild A549 cells, the expressions of COL1A1, COL1A5 and SOX4 were decreased in the miR-630-transfected A549 cells (P ＜0.01). The migration activity of A549 cells was decreased after transfected with miR-630 (P ＜0.01). The Luciferase activity of the SOX4-3 ′-UTR plasmid was significantly suppressed by miR-630 (P＜0.00). Conclusion The expression of miR-630 is down-regulated in lung adenocarcinoma; miR-630 inhibits migration and invasion of A594 cells, indicating that SOX4-3′-UTR may be its targeting gene. |
| Key words: Lung adenocarcinoma miR-630 Sex determining region Y-box 4 Invasion |
