| 摘要: |
| 目的观察槐耳清膏联合顺铂（DDP）作用于人胃腺癌SGC7901细胞后细胞周期的变化，并探讨其可能的作用机制。方法通过细胞流式实验，观察不同浓度槐耳清膏、DDP及两药联合作用于胃癌SGC7901细胞24h后细胞周期的变化情况，并通过Westernblot检测不同浓度的槐耳清膏、DDP及两药联合作用于胃癌SGC7901细胞后，细胞周期相关蛋白ATM、H2AX、cdc-2、CyclinB1等的表达情况。结果细胞流式实验结果显示，槐耳清膏能够阻滞SGC7901细胞于G2/M期，DDP能够阻滞SGC7901细胞于S期，而两药联用后S期和G2/M期都有阻滞。Westernblot实验结果显示，槐耳清膏、DDP均可上调p-ATM、γ-H2AX蛋白表达，同时下调CyclinB1蛋白表达，且两药联用后效果更加明显。结论槐耳清膏、DDP能够通过调节细胞周期相关蛋白的表达改变细胞周期，抑制细胞增殖，与槐耳清膏联用可提高DDP对胃癌的疗效。 |
| 关键词: 胃癌 槐耳 DDP 细胞周期 |
| DOI：10.12056/j.issn.1006-2785.2017.39.14.2016-2035 |
| 分类号: |
| 基金项目:浙江省重大科技专项计划项目（2013C03044-4） |
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| Combined effects of fungi Huaier extracts and cis-platin on cell cycle of human gastric cancer cell line SGC7901 |
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XIE Huaxia, XU Zhiyuan, ZHENG Guodian, HUANG Ting, ZHENG Guowei, TENG Fei, LV Hang, CHENG Xiangdong
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the First Affiliated Hospital of Zhejiang Chinese Medical University
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| Abstract: |
| Objective To investigate the combined effects of fungi Trametes robiniophila murr (Huaier) extracts and cis-platin (DDP) on cell cycle of gastric cancer cell line SGC7901in vitro and the underline mechanism. Methods Human gastric cancer SGC7901 cells were treated with Huaier and/or DDP in vitro. The cell cycle was examined by flow cytometry; and the expression of cell cycle-related proteins was detected by Western blot. Results The ratio of cells in G2/M phase of SGC7901 cells treated with Huaier were increased in a dose-dependent manner compared with control group; and the ratio of cells in S phase in DDP-treated groups were increased also in a dose-dependent manner. The cells arrested at G2/M and S phases were markedly increased in Huaier and DDP combined treatment groups. The expression of CyclinB1 was down-regulated and expression of p-ATM，酌-H2AX was up-regulated in Huaier and/or DDP treatment groups. Conclusion Huaier and DDP can change cell cycle through up-regulation of p-ATM, 酌- H2AX expression and down-regulation of CyclineB1 expression in human gastric cancer SGC7901 cells. |
| Key words: Gastric cancer Huaier DDP Cell cycle |
