| 摘要: |
| 目的探讨茶多酚对人前列腺癌PC-3M细胞侵袭力的影响。方法在人前列腺癌PC-3M细胞培养器皿中分别加入用完全培养液稀释的40、60、80滋g/ml茶多酚溶液，24、48h后通过细胞侵袭实验观察PC-3M细胞的侵袭能力，采用RT-PCR和Westernblot检测其MMP-2、TIMP-2mRNA、蛋白表达水平，并与只添加完全培养液的0滋g/ml组比较。结果茶多酚处理后的PC-3M细胞侵透基质胶，迁移至胶中或胶下表面的数量减少，且呈浓度、时间依赖性。与0滋g/ml组相比，40、60、80滋g/ml组MMP-2mRNA、蛋白表达水平均明显下调，TIMP-2mRNA、蛋白表达水平均明显上调；茶多酚可在mRNA、蛋白水平下调MMP-2、上调TIMP-2基因转录，大致呈浓度、时间依赖性（均P＜0.05）。结论茶多酚可在体外减弱人前列腺癌PC-3M细胞的侵袭力，可能与下调MMP-2、上调TIMP-2基因的转录与表达有关。 |
| 关键词: 茶多酚 前列腺癌 肿瘤侵袭 基质金属蛋白酶 -2 金属蛋白酶组织抑制因子 -2 |
| DOI：10.12056/j.issn.1006-2785.2017.39.12.2016-2210 |
| 分类号: |
| 基金项目: |
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| Effect of tea polyphenols on invasive ability of prostate cancer PC-3M cells |
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YAN Zhengqiang, CHEN Tieding, CHEN Li, ZHANG Qin, YANG Yuanqing
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Ningbo Medical Treatment Center Lihuili Hospital
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| Abstract: |
| Objective To investigate the effect of tea polyphenols (TPs) on invasive ability of human prostate cancer PC-3M cells. Methods Human prostate cancer PC-3M cells were treated with 40, 60, 80滋g/ml tea polyphenol for 24h or 48h in vitro. The invasive ability of PC-3M cells was determined by Transwell assay, and the expression of MMP-2, TIMP-2 mRNA and protein was detected by RT-PCR and Western blot, respectively. Results Transwell assay showed that the invasive ability of PC-3M cells was decreased after treatment with tea polyphenol. The expression of MMP-2 mRNA and protein in PC-3M cells
was increased after treated with 60滋g/ml and 80滋g/ml for 24h or 48h were significantly decreased (P＜0.05), while the expression of TIMP-2 mRNA and protein was increased (P＜0.05) in a dose-dependent and time-dependent manner (P＜0.05). Conclusion Tea polyphenols can reduce the invasive ability of human prostate cancer PC-3M cells in vitro, which may be related to the down-regulation of MMP-2 and up-regulation of TIMP-2 gene expression. |
| Key words: Tea polyphenol Prostate cancer Neoplasm invasiveness MMP-2 TIMP-2 |
