| 摘要: |
| 目的探讨血清、关节液骨桥蛋白（OPN）在幼年特发性关节炎（JIA）中的临床意义。方法采集确诊为JIA39例患儿（JIA组）及腹股沟斜疝修补术30例患儿（对照组）的静脉血，并抽取JIA组急性期15例患儿的关节液，采用ELISA法测定血清、关节液OPN水平。结果JIA组血清OPN水平急性期[（4.8±2.4）ng/ml]明显高于缓解期[（2.1±1.2）ng/ml]及对照组[（0.5±0.1）ng/ml]，差异有统计学意义（P＜0.05）。JIA组关节液OPN水平[（16.5±1.7）ng/ml]明显高于血清OPN水平[（4.8±2.4）ng/ml]，差异有统计学意义（t=17.234，P＜0.05）。JIA组中关节破坏的患儿血清OPN水平[（6.9±1.9）ng/ml]明显高于无关节破坏的患儿[（3.2±1.2）ng/ml]，差异有统计学意义（t=7.243，P＜0.05）。JIA组急性期血清OPN水平与血白细胞、中性粒细胞、C反应蛋白、血沉、关节肿胀数呈正相关（r=0.662、0.811、0.777、0.833、0.375，均P＜0.05），与血红蛋白、血小板、关节压痛数、晨僵时间无关（r=0.091、0.296、0.229、0.019，均P＞0.05）。结论OPN可能作为促炎因子参与JIA疾病活动、关节破坏。 |
| 关键词: 骨桥蛋白 关节液 幼年特发性关节炎 关节破坏 |
| DOI： |
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| 基金项目:浙江省宁波市科技局科研项目；浙江省宁波市卫生局科研项目；浙江省重点平台科研项目 |
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| Clinical significance of serum and joint fluid osteopontin levels in juvenile idiopathic arthritis |
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YU Hanchun,WU Ling,ZHONG Shiling,CHEN Yuanling,DI Yazhen,WANG Jiapei
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Ningbo Women and Children's Hospital
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| Abstract: |
| Objective To evaluate the clinical significance of osteopontin (OPN) in serum and joint fluid of patients with juvenile idiopathic arthritis (JIA). Methods Thirty nine children with JIA in acute phase or remission phase and 30 children with inguinal hernia(control group) were enrolled in this study. Joint fluid was extracted in 15 JIA patients in acute phase. Enzyme linked immunosorbent assay (ELISA) was employed to measure the level of OPN in serum and joint fluid. Results Serum OPN level of JIA children in acute phase(4.8±2.4 ng/ml) was significantly higher than that of control group (0.5 ± 0.1ng/ml) and JIA children in the remission stage (2.1±1.2 ng/ml)(all P＜0.05). The joint fluid OPN level of JIA children in acute phase was significantly higher than serum OPN level of JIA children in acute phase (16.5±1.7 ng/ml vs 4.8±2.4ng/ml, P＜0.05). The serum OPN level of JIA with joint damagewas significantly higher than that without joint destruction(6.9±1.9 ng/ml vs 3.2±1.2 ng/ml, P＜0.05). The serum OPN level of JIA children in acute phase was positively correlated with WBC, neutrophil, CRP, ESR, the number of swelling joints (r=0.662, 0.811, 0.777, 0.833 and 0.375, all P＜0.05), while not correlated with hemoglobin, platelet, the number of painful joint, morning stiffness(r=0.091, 0.296, 0.229 and 0.019, all P ＞0.05). Conclusion OPN may be as a proinflammatory factor involved in JIA activity and joint damage. |
| Key words: Osteopontin Joint fluid Juvenile idiopathic arthritis Joint damage |
