| 摘要: |
| 目的探讨高危型人乳头瘤病毒（HPV）E6/E7mRNA检测对高级别宫颈病变的临床诊断价值。方法选取2014年8月至2015年3月在妇科门诊及住院的疑似宫颈病变患者311例，均行宫颈脱落细胞的薄层液基细胞学检查、HPVDNA分型检测和高危型HPVE6/E7mRNA检测，对其中任意一项或多项结果阳性者行阴道镜下宫颈活组织检查和组织病理学检查。以病理报告为金标准，进行统计学分析。结果慢性宫颈炎组中HPVDNA分型检测检出率（29.4%）高于高危型HPVE6/E7mRNA（17.5%），差异有统计学意义（P＜0.05）。高危型HPVE6/E7mRNA检测预测高级别宫颈病变的特异度（73.4%）高于HPVDNA分型检测（59.8%），差异有统计学意义（P＜0.05）。高危型HPVE6/E7mRNA检测诊断高级别宫颈病变ROC曲线下面积（0.816）大于HPVDNA分型检测（0.710），差异有统计学意义（P＜0.05）。结论与HPVDNA分型检测相比，高危型HPVE6/E7mRNA检测预测高级别宫颈病变具有更好的特异度，其诊断效能更佳。 |
| 关键词: 人乳头瘤病毒 E6/E7 mRNA DNA 宫颈上皮内瘤样病变 宫颈肿瘤 |
| DOI： |
| 分类号: |
| 基金项目:温州市公益性科技计划项目 |
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| Clinical value of high-risk HPV E6/E7 mRNA in detection of high-grade cervical lesions |
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ZHENG Zhi,CAO Xiaoen,ZHANG Hongping
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Wenzhou People's Hospital
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| Abstract: |
| Objective To assess the clinical value of high-risk human papilloma virus (HPV) E6/E7 mRNA in detection of high-grade cervical lesions (cervical intraepithelial neoplasm II, III and cervical cancer). Methods A total of 311 patients with suspected cervical lesions were visiting our hospital between August 2014 and March 2015 were included. Thinprep cytology test (TCT) of exfoliated cervical cells, HPV DNA classification detection and high-risk HPV E6/E7 mRNA detection were performed for all patients; patients with positive results of one or multiple tests underwent colposcopic cervical biopsy and histopathological examination. With pathological result as the gold standard, the clinical value of two tests was analyzed and compared. Results The positive rate of chronic cervicitis with HPV DNA classification was higher than that with high-risk HPV E6/E7 mRNA detection (29.4% vs. 17.5%, P ＜0.05). The specificity of high-risk HPV E6/E7 mRNA test in detecting high-grade cervical lesions was higher than that of HPV DNA classification test (73.4% vs 59.8%, P＜0.05). The area under the ROC curve of high-risk HPV E6/E7 mRNA detection in diagnosing high-grade cervical lesions was larger than that of HPV DNA classification test (0.816 vs. 0.710,
P＜0.05). Conclusion Compared to HPV DNA classification test, high-risk HPV E6/E7 mRNA detection has better clinical value in prediction of high-grade cervical lesions. |
| Key words: Human papillomavirus E6/E7 mRNA DNA Cervical intraepithelial neoplasia Cervical carcinoma |
