| 摘要: |
| 目的探讨腺病毒介导的肿瘤生长抑制因子4（Ad-ING4）在体外对人胃癌AGS细胞凋亡的作用及其p53基因表达的影响。方法不同浓度（80、160、320ng/ml）的Ad-ING4作用人胃癌AGS细胞48h后，用AnnexinV-FITC检测细胞凋亡率，RT-PCR检测p53mRNA的表达，免疫印迹法检测p53蛋白表达水平，并与对照组进行比较。结果凋亡检测发现，不同浓度Ad-ING4可明显诱导人胃癌AGS细胞凋亡，并呈浓度依赖性（P＜0.05）；Ad-ING4作用前AGS细胞p53mRNA和p53蛋白表达水平较低，不同浓度Ad-ING4作用后p53mRNA和p53蛋白表达水平明显上调，呈浓度依赖性（P＜0.05）。结论随Ad-ING4作用浓度增高，人胃癌AGS细胞出现明显凋亡，其机制可能与p53基因转录活性增强有关。 |
| 关键词: 实验性自身免疫性重症肌无力 骨髓间充质干细胞 基质金属蛋白酶 9 |
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| Treatment of experimental autoimmune myasthenia gravis with bone marrow stromal stem cells in rats |
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CHENG Haiyan, SHEN Zhe
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Shaoxing Second Hospital
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| Abstract: |
| Objective To investigate the effects of bone marrow stromal stem cells (BMSCs) in treatment of experimental autoimmune myasthenia gravis (EAMG) in rats. Methods Thirty Lewis rats were divided randomly into the transplantation group, the model control group and the normal control group with 10 rats in each group. EAMG model was induced by intraperitoneal injection of mAb-35 in transplantation group and model control group. BMSCs were isolated, cultured and identified from healthy Lewis rats. Rats in transplantation group were injected with 1.5×106 BMSCs diluted in 600ml PBS, and the same volume of PBS was injected in the model control and the normal control group. The symptoms of rats were observed, the titers of plasma AChR-Ab were detected by ELISA, the AChR changes in gastrocnemius were examined by immunofluorescence technique, and the expressions of AChR and MMP-9 mRNA were detected by real-time RT-PCR in all groups. Results The clinical score, the titers of AChR-Ab, and the expression of AChR mRNA in the transplantation group were significantly lower than those in the model control group. There were no significant differences in above variates between transplantation and the normal control
group. The immunofluorescence assay showed EAMG group had no red fluorescence, while the transplantation group had intermittently weak fluorescence. The expression of MMP-9 mRNA was higher in the model control group. However, there was no significant difference between transplantation group and normal control group. Conclusion BMSCs can ameliorate clinical manifestations in EAMG rats, which may be associated with inhibition of MMP-9 expression. |
| Key words: Experimental autoimmune myasthenia gravis Bone marrow stromal cells Matrix metalloproteinase 9 |
