| 摘要: |
| 目的探讨微囊蛋白1、细胞内钙离子在转化生长因子茁1（TGF-茁1）诱导哮喘大鼠气道平滑肌细胞（ASMCs）增殖中的作用。方法离体培养大鼠ASMCs，并分为正常组、哮喘组、TGF-茁1干预组、TGF-茁1+β-环糊精（茁-CD）干预组，CCK-8法检测各组细胞的增殖水平；Westernblot法检测各组微囊蛋白1含量；流式细胞仪检测各组ASMCs胞内钙离子荧光强度。结果哮喘组OD值较正常组明显增加（P＜0.05），TGF-茁1组OD值较正常组、哮喘组明显增加（P＜0.05），TGF-茁1+茁-CD组OD值较TGF-茁1组明显增加（P＜0.05）。TGF-茁1组较正常组和哮喘组微囊蛋白1含量减少（P＜0.05），TGF-茁1+茁-CD组较TGF-β1组微囊蛋白1含量减少（P＜0.05）。TGF-茁1组较正常组和哮喘组胞内钙离子荧光强度增加（P＜0.05），TGF-茁1+茁-CD组较TGF-茁1组胞内钙离子荧光强度增加（P＜0.05）。相关关系分析示细胞增殖水平与微囊蛋白1含量呈负相关（r=-0.51，P＜0.05），细胞增殖水平与钙离子荧光强度呈正相关（r=0.60，P＜0.05），微囊蛋白1含量与钙离子荧光强度呈负相关（r=-0.87，P＜0.05）。结论微囊蛋白1可以抑制TGF-茁1诱导的ASMCs增殖,这种抑制作用可能部分是通过减少胞内游离钙离子来实现的。 |
| 关键词: 哮喘 平滑肌细胞 增殖 微囊蛋白 转化生长因子 茁1 钙离子 茁 环糊精类 大鼠 |
| DOI： |
| 分类号: |
| 基金项目:浙江省医药卫生科技计划 |
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| Effect of caveolin-1 and intracellular Ca2+ on the TGF-β1-induced ASMCs proliferation in asthmatic rats |
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WANG Ruili, DAI Wei, LI Fengqin, WU Liqin, DAI Yuanrong
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the Second Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the effect of caveolin-1 and intracellular Ca2+ ([Ca2+] (i)) on TGF-β1-induced
proliferation of airway smooth muscle cells(ASMCs) in asthmatic rats. Methods ASMCs of rats were isolated and cultured in vitro. CCK-8 method was used to detect cell proliferation and Western blot method was used to detect the expression of caveolin-1 protein. FCM was used to detect [Ca2+](i). Results OD value in asthma group was higher than that in normal group (P＜0.05), OD value in TGF-β1 group was higher than that in normal group and asthma group (P＜0.05), OD value in TGFβ1+β-CD group was higher than that in TGF-β1 group (P＜0.05). The expression of caveolin-1 in TGF-β1 group was significantly lower than that in normal group and asthma group (P＜0.05), the expression of caveolin-1 in TGF-β1+ β-CD group was significantly lower than that in TGF-β1 group (P＜0.05). The [Ca2+](i) of ASMCs in TGF-β1 group was higher than that in normal group and asthma group(P＜0.05), the[Ca2+](i) of ASMCs in TGF-β1+ β-CD group was higher than that in TGF-β1 group(P＜0.05). There was negative correlation between cell proliferation and the expression of caveolin-1 protein (r=-0.51, P＜0.05), cell proliferation was positively correlated with the [Ca2+](i) (r=0.60, P＜0.05), caveolin-1 protein was negatively correlated with the [Ca2+](i)(r=-0. 87, P ＜0.05). Conclusion Caveolin-1 cab suppress TGF-β1-induced ASMCs proliferation in asthmatic rats, which is
associated with the decrease of [Ca2+](i). |
| Key words: Asthma Airway smooth muscle cells Proliferation Caveolins TGF-茁1 Ca2+ Beta-cyclodextrins Rats |
