| 摘要: |
| 目的应用微滴数字PCR检测血清中miR-21和miR-4429的水平，并探讨联合AFP检测在诊断肝细胞肝癌中的临床价值。方法收集69例肝癌、47例慢性活动性乙肝以及40例健康体检者血清标本，应用微滴数字PCR检测血清miR-21和miR-4429水平，化学发光法检测血清中AFP水平，并用ROC曲线和Spearman相关进行分析。结果肝癌组患者miR-21水平明显高于健康对照组和慢性活动性乙肝组（均P＜0.05），miR-4429水平高于健康对照组（P＜0.05）。ROC曲线分析显示：miR-21、miR-4429和AFP3者联合检测的灵敏度（95.0%）、特异度（92.5%）和准确度（92.7%），较单独检测或两项联合检测有较大提升。miR-21、miR-4429表达与患者年龄、性别、有无HBsAg感染或肝硬化、PLT或AFP水平、TNM分期和疾病状态均无相关性（均P＞0.05）。结论血清miR-21和miR-4429对肝癌的诊断具有良好的灵敏度和特异度，联合AFP检测可以提高诊断的效能。 |
| 关键词: 微滴数字 PCR 肝细胞肝癌 miR-21 miR-4429 |
| DOI：10.12056/j.issn.1006-2785.2017.39.03.2016-408 |
| 分类号: |
| 基金项目:浙江省医药卫生一般研究计划项目资助（2012KYA183）；台州市市级科技资金项目（121KY09-7）；台州市市级科技资金项目(14SF04) |
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| Serum miR-21 and miR-4429 levels detected by droplet digital PCR for diagnosis of hepatocellular carcinoma |
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WANG Pan, MO Jingang, ZHU Jie, LI Zhaoyun
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Taizhou Central Hospital
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| Abstract: |
| Objective To investigate the serum miR-21 and miR-4429 levels detected by droplet digital PCR (ddPCR) in diagnosis of hepatocellular carcinoma (HCC). Methods Serum miR-21 and miR-4429 levels were determined by ddPCR in 69 HCC patients, 47 patients with chronic active hepatitis B (CHB) and 40 healthy subjects. Serum AFP levels were measured by chemiluminescence method. ROC curve and Spearman rank correlation were performed data analysis. Results Serum miR-21 levels of HCC group were increased significantly compared with the control group and CHB group (P＜0.05), serum miR-4429 levels increased significantly compared with the normal control group (P＜0.05). ROC curves showed that the sensitivity (95%), specificity (92.5%) and accuracy(92.7%) of combination of miR-21, miR-4429 and AFP were higher than those of single test or two joint tests. Serum miR-21 and miR-4429 levels were not correlated with age, sex, HBsAg, platelets count, AFP, liver cirrhosis, TNM and disease status (P ＞0.05). Conclusion miR-21 and miR-4429 show a good sensitivity and specificity for diagnosis of HCC, and the combination with AFP may further improve the diagnostic efficiency. |
| Key words: ddPCR Hepatocellular carcinoma miR-21 miR-4429 |
