| 摘要: |
| 目的探讨茶多酚对人前列腺癌PC-3M细胞中调控细胞凋亡的生存素（Survivin）mRNA和蛋白表达的影响。方法在人前列腺癌PC-3M细胞培养器皿中分别加入用完全培养液稀释的0、40、60、80滋g/ml茶多酚溶液，24、48h后应用RT-PCR和Westernblot检测并比较SurvivinmRNA、蛋白表达水平。结果与0滋g/ml组相比，40滋g/ml组加入茶多酚48h后SurvivinmRNA、蛋白表达水平均明显下调（均P＜0.05）；60、80滋g/ml组加入茶多酚24、48h后SurvivinmRNA、蛋白表达水平均明显下调（均P＜0.05），其中80滋g/ml组下调最为明显（均P＜0.05）；大致呈剂量、时间依赖性（均P＜0.05）。结论茶多酚可下调SurvivinmRNA和蛋白表达水平，促进人前列腺癌PC-3M细胞调亡，在一定范围内呈剂量、时间依赖性。 |
| 关键词: MMP12 mRNA Lung adenocarcinoma Histopathological subtypes Gender |
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| Effect of tea polyphenols on mRNA and protein expression of survivin in prostate cancer PC-3M cells |
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YAN Zhengqiang, YANG Yuanqing, LU Qihai
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Ningbo Medical Treatment Center Lihuili Hospital
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| Abstract: |
| Objective To investigate the effect of tea polyphenols on the mRNA and protein expression of survivin in prostate cancer PC-3M cells. Methods Human prostate cancer PC-3M cells were treated with 40, 60, 80滋g/ml tea polyphenol for 24h or 48h, the mRNA and protein expression of survivin in PC-3M cells were detected by RT-PCR and western blot, respectively. Results The mRNA and protein expression of survivin in PC-3M cells treated with 60滋g/ml and 80滋g/ml for 24h and 48h was significantly decreased in a dose-dependent and time-dependent manner(P＜0.05). Conclusion Tea polyphenols can promote apoptosis of human prostate cancer PC-3M cells, which is related to the down-regulation of survivin gene tran- scription and translation. |
| Key words: Tea polyphenol Prostate cancer PC-3M cells Apoptosis Survivin |
