| 摘要: |
| 目的构建携带人HGFK1基因的真核表达载体pcDNA3.1（-）-HGFK1。方法通过PCR方法从已经构建好的病毒穿梭载体中扩增出HGFK1基因，构建pcDNA3.1（-）-HGFK1真核表达载体，经PCR、酶切、测序、蛋白表达等方法进行鉴定。结果PCR、酶切、测序以及蛋白表达证实pcDNA3.1（-）-HGFK1载体序列正确。结论本研究成功构建了pcDNA3.1（-）-HGFK1真核表达载体，可为后续进行肝癌细胞的生物学研究提供帮助。 |
| 关键词: 基因 HGFK1 载体构建 |
| DOI： |
| 分类号: |
| 基金项目:浙江省医药卫生科技计划项目；浙江省科技计划项目 |
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| Construction and identification of pcDNA3.1(-)-HGFK1 plasmid |
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ZHU Yu, GU Lingling, ZhU Min, ZHANG Fanbiao, FANG Zheping
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Taizhou Hospital of Zhejiang Province
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| Abstract: |
| Objective To construct human pcDNA3.1(-)-HGFK1 gene eukaryotic expression vector. Methods HGFK1 gene was amplified by PCR method from the constructed virus shuttle vector, and the pcDNA3.1 (-) -HGFK1 eukaryotic expression vector was constructed and identified by PCR, enzyme digestion, sequencing and protein expression. Results Enzyme digestion, PCR, sequencing and protein expression showed the successful construction of pcDNA3.1 (-)-HGFK1 eukaryotic expression vector. Conclusion We construct pcDNA3.1(-)-HGFK1 eukaryotic expression vector successfully, which may be used for further study of hepatocellular carcinoma gene therapy. |
| Key words: Gene HGFK1 Vector Construction |
