| 摘要: |
| 目的研究人参皂甙Rg3对胰腺癌SW-1990细胞皮下移植瘤生长及血管生成拟态的影响。方法用胰腺癌SW-1900细胞株建立裸鼠胰腺癌皮下移植瘤模型，分成4组：0.9%氯化钠溶液对照组、5mg/kg人参皂甙Rg3组、10mg/kg人参皂甙Rg3组、20mg/kg人参皂甙Rg3组，每组7只。各组裸鼠予腹腔注射给药，隔天1次，给药4周。给药结束后3d处死裸鼠，检测各组移植瘤的体积和重量；CD31/PAS染色观察移植瘤血管生成拟态的变化；荧光定量PCR和Westernblot检测各组移植瘤钙粘蛋白（VE-Cadherin）、络氨酸激酶受体2（EphA2）、基质金属蛋白2（MMP-2）、基质金属蛋白9（MMP-9）mRNA和蛋白表达水平。结果与对照组比较，20mg/kg人参皂甙Rg3组裸鼠胰腺癌皮下移植瘤体积减小、重量减轻（均P＜0.05）。与对照组比较，5mg/kg人参皂甙Rg3组、10mg/kg人参皂甙Rg3组、20mg/kg人参皂甙Rg3组裸鼠胰腺癌皮下移植瘤拟态、正常血管生成均减少（均P＜0.05），VE-Cadherin、EphA2、MMP-2、MMP-9mRNA和蛋白表达水平均降低（均P＜0.05）。结论人参皂甙Rg3可抑制胰腺癌SW-1900细胞皮下移植瘤生长和血管生成拟态形成，可能是通过调控VE-Cadherin、EphA2、MMP-2、MMP-9的表达发挥抗肿瘤作用。 |
| 关键词: 裸鼠皮下移植瘤 血管生成拟态 钙粘蛋白 络氨酸激酶受体 2 基质金属蛋白 9 基质金属蛋白 2 |
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| Effect of ginsenoside Rg3 on growth and vasculogenic mimicry of implanted human pancreatic cancer in nude mice |
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GUO Jingqiang, LIN Shengzhang
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Lishui Central Hospital
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| Abstract: |
| Objective To investigate the effects of ginsenoside Rg3 on growth and vasculogenic mimicry of implanted human pancreatic cancer in nude mice. Methods Human pancreatic cancer SW-1990 cells were inoculated subcutaneously in nude mice. The tumor bearing nude mice were divided into four groups with 7 mice in each group. The animals were injected intraperitoneally with saline control, 5 mg/kg, 10mg/kg and 20mg/kg ginsenosides Rg3 for 4 wk, respectively. The animals were sacrificed 3d after completion of the treatment. The weight and size of xenografts were measured, the vasculogenic mimicry was observed by immunohistochemistry with CD31/PAS staining, and the mRNA/protein expressions of VE-Cadherin, EphA2, MMP-2, MMP-9 were detected by fluorescent RT-PCR and Western blot, respectively. Results Ginsenosides Rg3 significantly inhibited weight and size of transplanted tumors; and inhibited vasculogeic mimicry. The expression of VE-Cadherin, EphA2, MMP-2 and MMP-9 mRNA and protein were down-regulated. Conclusion Rg3 can inhibit vasculogenic mimicry of transplanted human pancreatic cancer in nude mice, which may be associated with the down-regulation of VE-Cadherin, EphA2, MMP9 and MMP2 expression. |
| Key words: Subcutaneous transplantation tumor Vasculogenicmimicry VE-cadherin EphA2 MMP-2 MMP-9 |
