| 摘要: |
| 目的探讨肌聚糖蛋白α（α-SG）、肌聚糖蛋白β（β-SG）在良性前列腺增生症（BPH）患者膀胱逼尿肌中的表达。方法选择67例住院手术的BPH患者为研究对象，根据排尿后膀胱残余尿量分为尿潴留组31例（＞50ml）和非尿潴留组36例（＜50ml），并选择同期住院、无BPH且排尿后膀胱内无残余尿的男性膀胱恶性肿瘤患者28例为对照组；采集患者逼尿肌标本，采用免疫组化、Westernblot法检测α-SG、β-SG表达。结果免疫组化显示α-SG、β-SG免疫阳性产物呈棕黄色颗粒，主要位于细胞质，与膀胱逼尿肌细胞走向一致。与对照组相比，尿潴留组α-SG、β-SG表达水平均明显下降，非尿潴留组均明显增加，差异均有统计学意义（均P＜0.05）。结论SG在BPH患者膀胱逼尿肌中存在异常表达，可能与BPH临床进展有关。 |
| 关键词: 肌聚糖蛋白 前列腺增生 尿潴留 |
| DOI： |
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| 基金项目:温州市公益性科技计划项目 |
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| Expression of sarcoglycans in detrusor of bladder in patients with benign prostatic hyperplasia |
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MU Haiqi, ZHU Shijian, YANG Sen, NAN Cunjin, CHEN Yinghe
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the Second Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the expression of α-sarcoglycan (α-SG) and 茁-sarcoglycan (茁-SG) in detrusor of bladder in patients with benign prostatic hyperplasia (BPH) Methods Sixty seven patients with BPH were enrolled in the study, including 31 cases with urinary retention (＞50ml), and 36 cases without urinary retention (＜50ml), and 28 bladder tumor patients without BPH and urinary retention as control group. The expressions of α-SG, 茁-SG in detrusor tissue were detected by immunohistochemical technique and Western blot. Results Immunohistochemistry showed that positive staining of α-SG, β-SG was mainly present in the cytoplasm, and arrayed along bladder detrusor cells. Compared to the control group, the expression of α-SG, β-SG in detrusor in urinary retention group was decreased (P＜0.05), while the expression in non-urinary
retention group was increased (P＜0.05). Conclusion The abnormal expression of sarcoglycans in the detrusor of bladder is associated with the clinical progress of BPH patients. |
| Key words: Sarcoglycan Benign prostatic hyperplasia Urinary retention |
