| 摘要: |
| 目的研究核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）炎性体相关分子表达与老年2型糖尿病（T2DM）的关系。方法采用ELISA法测定30例老年T2DM患者、30例健康体检者（对照组）血浆中IL-1β、IL-18和IL-37水平，分别采用定量聚合酶链反应（PCR）技术和ELISA技术检测糖尿病患者单核细胞来源的巨噬细胞（MDM）表达NLPR3、凋亡相关斑点样蛋白（ASC）和IL-1βmRNA水平及葡萄糖诱导MDM分泌IL-1β和TNF-琢的水平。结果老年T2DM患者血浆中的IL-1β和IL-18水平均显著高于对照组，差异均有统计学意义（均P＜0.05）；而IL-37无统计学差异；T2DM患者MDM中NLPR3、ASC和IL-1βmRNA表达水平均显著高于对照组，差异均有统计学意义（均P＜0.05）；且经葡萄糖刺激后IL-1β、TNF-琢水平显著高于对照组，差异有统计学意义（均P＜0.05）。结论NLRP3炎性体相关分子的表达与炎性体活化可能参与老年T2DM的炎症反应过程。 |
| 关键词: 白细胞介素 -1β 2 型糖尿病 核苷酸结合寡聚化结构域样受体蛋白 3 炎性体 肿瘤坏死因子 |
| DOI： |
| 分类号: |
| 基金项目:浙江省医药卫生科技计划项目 |
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| Expression of NLRP3 inflammasome-related molecules in elderly patients with type 2 diabetes mellitus |
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HONG Baojian, SU Liyun, LIANG Lijuan, ZHU Yuexia, XU Fang, CHEN Jianzhong
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Zhejiang provincial People's Hospital
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| Abstract: |
| Objective To investigate the expression of NLRP3 inflammasome-related molecules in elderly patients with type 2 diabetes mellitus (T2DM) . Methods The plasma levels of IL-1β, IL-18 and IL-37 in 30 T2DM patients and 30 healthy controls were measured with ELISA; the expression of NLRP3, ASC and IL-1β in monocytes derived macrophages (MDM) were detected by real time PCR. The contents of IL-1β and TNF-琢 secreted by MDM treated by glucose were also analyzed. Results Plasma IL-1β and IL-18 levels in T2DM patients was significantly higher than those in healthy controls (P＜0.05), while IL-37 level was no significantly increased (P ＞0.05). Expression of NLRP3, ASC and IL-1 in MDM and IL-1β, TNF-琢 secreted by MDM were significantly higher than those in controls (P＜0.05). Conclusion The NLRP3 inflammasome pathway may be involved
in the inflammatory process of elderly patients with T2DM. |
| Key words: IL-1β T2DM NLRP3 Inflammasomes TNF |
