| 摘要: |
| 目的探讨中国汉族人群基质金属蛋白酶-3（MMP-3）基因多态性与无症状性颈动脉狭窄的关系。方法选择2011年1月至2013年12月来院体检的425例年龄≥60岁健康者为研究对象，经颈动脉超声检测，其中颈动脉狭窄（≥50%）65例，无颈动脉狭窄360例；并采用聚合酶链反应-限制性酶切片段长度多态性方法，分析与比较两组人群MMP-3基因启动子区-1171位点的5A/6A多态性。结果颈动脉狭窄与无狭窄人群基因型分布均符合Hardy-Weinberg平衡（均P＞0.05）；两组人群MMP-35A/6A基因型和5A等位基因分布比较，差异均有统计学意义（均P＜0.05）。经logistic回归模型分析，校正多项血管危险因素后，5A/6A或5A/5A基因型是无症状性颈动脉狭窄的独立危险因素（OR=1.98，95%CI：1.05~3.72）。结论MMP-3基因启动子区-1171位点的5A/6A多态性与无症状性颈动脉狭窄的发生可能相关。 |
| 关键词: 基质金属蛋白酶 -3 基因多态性 无症状性颈动脉狭窄 |
| DOI： |
| 分类号: |
| 基金项目:浙江省温州市科技计划项目 |
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| Association between matrix metalloproteinase-3 5A/6A polymorphism and prevalence of asymptomatic carotid artery stenosis |
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HUANG Xiaoya, YAN Liya, HUANG Xiangdong, GUAN Chaohong, MAO Xinlei, LIN Caidan
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Wenzhou Central Hospital and Dingli Clinical institute of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the relationship between matrix metalloproteinase-3 (MMP-3) -1171 5A/6A polymorphism and the prevalence of asymptomatic carotid artery stenosis in Han Chinese population. Methods Total 425 healthy subjects aged ≥60 years were enrolled in this study from January 2011 to December 2013. The genotypes of MMP-3 promoter polymorphism (-1171 5A/6A) were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results Among 425 subjects 65 showed evidence of asymptomatic carotid artery stenosis. The
genotype distributions of the asymptomatic carotid artery stenosis group and the non-stenosis group were both in Hardy-Weinberg equilibrium (P ＞0.05). The frequencies of MMP-3 5A/6A+5A/5A genotype and 5A allele in the asymptomatic carotid artery stenosis group was significantly higher than those in non-stenosis group(P＜0.05). After adjustment for vascular risk factors, multivariate logistic regression analysis showed that the MMP-3 5A/6A+5A/5A genotype was an independent risk factor for asymptomatic carotid artery stenosis (OR=1.98, 95%CI: 1.05-3.72). Conclusion The MMP-3 (-1171 5A/6A) polymorphism may contribute to susceptibility of asymptomatic carotid artery stenosis. |
| Key words: Matrix metalloproteinase-3 Polymorphism asymptomatic Asymptomatic carotid antery stenosis |
