| 摘要: |
| 目的观察山竹提取物（GME）对人胃癌细胞SGC-7901增殖情况的影响，探讨其作用机制。方法采用新型四氮唑盐法（MTS）检测GME对SGC-7901细胞增殖的影响；采用流式细胞仪检测GME对SGC-7901细胞凋亡和诱导活性氧（ROS）水平的影响；采用Westernblot检测不同浓度GME对BGC-7901细胞的PTEN、Bax、Bcl-2表达的影响。结果GME显著抑制SGC-7901细胞的增殖（P＜0.05），与阳性对照组5-氟尿嘧啶（5-FU）相比抑制率也较高（P＜0.05），其半数抑制浓度为：7.89滋g/ml。经5、7.5滋g/ml的GME处理24h后，可有效促进SGC-7901细胞凋亡（P＜0.05），ROS水平的累积；GME相同方式处理后，SGC-7901细胞中PTEN和Bax的蛋白表达量上调，Bcl-2蛋白表达量降低（P＜0.05）。结论GME能够显著抑制胃癌SGC-7901细胞的增殖，能够诱导SGC-7901细胞ROS水平的累积，并通过影响PTEN、Bax和Bcl-2蛋白的表达，促进其凋亡。 |
| 关键词: 山竹提取物 增殖 胃癌 SGC-7901 细胞 |
| DOI： |
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| 基金项目:浙江省中医药优秀青年人才基金 |
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| Effect of Garcinia mangostana extract on proliferation of gastric cancer SGC-7901 cells in vitro |
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WANG Rongguo,LI Kaiqiang,ZHANG Zhijian,WANG Qiang
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Taizhou First People's Hospital
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| Abstract: |
| Objective To investigate the effect of Garcinia mangostana Extract (GME) on proliferation of gastric cancer SGC-7901 cells. Methods Human gastric cancer SGC-7901 cells were treated with 5滋g/ml and 7.5滋g/ml GME for 24h. SGC-7901 cell proliferation was determined by tetrazolium salt reduction method (MTS), cell apoptosis and reactive oxygen species (ROS) were measured by flow cytometry, PTEN, Bax and Bcl-2 protein expressions in SGC-7901 cells were detected by Western blot. Results GME significantly inhibited the proliferation of SGC-7901 cells(P＜0.05) witha 50% inhibitory concentrations
of 7.89滋g/ml, the inhibition rate was higher than that of 5-fluorouracil (5-FU)(P＜0.05). After treated with 5 μg/ml and 7.5滋g/ml of GME for 24h, apoptosis of SGC-7901 cells was increased (P＜0.05 ), and the cell ROS levels was also increased; the expressions of PTEN and Bax proteins in SGC-7901 cells were increased, while Bcl-2 expression levels were reduced (P＜0.05). Conclusion This study suggests that GME can significantly inhibit proliferation of SGC-7901 cells, enhance ROS levels, and promote apoptosis of SGC-7901 cells, which is associated with up-regulation of PTEN, Bax and down-regulation of Bcl-2 expression. |
| Key words: Garcinia mangostana extract Proliferation Gastric cancer SGC-7901 cell line |
