| 摘要: |
| 目的探讨水杨酸抑制人胃癌细胞侵袭转移的分子机制。方法不同浓度水杨酸体外处理胃癌细胞株MGC-803后，采用基质胶黏附试验、迁移试验和Transwell侵袭试验检测胃癌细胞黏附、迁移和侵袭能力的变化；采用荧光定量PCR、Westernblot检测细胞黏附相关分子核纤层蛋白（Lamin）、核纤层蛋白C1（LaminC1）、核纤层蛋白B1（LaminB1）、纤连蛋白（FN1）、CD44、整合素A9（ITGA9）、连环蛋白A1（CTNNA1）和E钙粘素（CDH1）表达水平的变化。结果与空白对照比较，0.5mmol/L和1mmol/L水杨酸体外处理胃癌细胞24h后，胃癌细胞的增殖、黏附、迁移和侵袭能力均受抑制（均P＜0.05）；Lamin、LaminC1、LaminB1、FN1、CD44、ITGA9和CTNNA1mRNA表达水平均下降（均P＜0.05），CDH1mRNA表达水平均升高（均P＜0.05）；LaminB1、FN1、CD44和CTNNA1蛋白表达水平均升高（均P＜0.05）。结论一定浓度的水杨酸可体外抑制胃癌细胞增殖、黏附、侵袭能力，从而抑制胃癌转移，其作用机制可能与调节细胞黏附相关分子的表达水平和抑制胃癌细胞上皮间质转化有关。 |
| 关键词: 水杨酸 胃癌细胞株 侵袭转移 细胞黏附分子 |
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| Effect of salicylic acid on adhesion, migration and invasion of human gastric cancer cells |
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GE Shuxiong, LIU Yahui, ZHANG Li, LI Keshi, HU Yue, WANG Yong
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the Affiliated Hospital,College of Medicine, Ningbo University
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| Abstract: |
| Objective To investigate the effects of salicylic acid on adhesion, migration and invasion abilities of gastric cancer cell line and their mechanisms. Methods Human gastric cancer cells were incubated with different concentrations of salicylic acid. The abilities of adhesion, migration and invasion of gastric cancer cells were evaluated with cell adhesion assay, Transwell migration assay and Transwell invasion assay. The mRNA and protein expression levels of cell adhesion moleculars were tested by Real-time RT- PCR and Western blot, respectively. Results Salicylic acid significantly inhibited the proliferation, adhesion, migration and invasion of gastric cancer cells in vitro. The mRNA expression levels of Lamin, LaminC1, LaminB1, FN1, CD44, ITGA9 and CTNNA1 were decreased; however, expression of CDH1 was increased after salicylic acid treatment. While the
protein expression levels of LaminB1, FN1, CD44 and CTNNA1 were also decreased(P＜0.05). Conclusion Salicylic acid inhibits
the proliferation, adhesion, migration and invasion of gastric cancer cell, which is associated with the expression of cell adhe- sion-related molecules and the resistance to epithelial-mesenchymal transition. |
| Key words: Salicylic acid Gastric cancer cell line Migration and invasion Cell adhesion molecules |
